Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker (ab8135)

Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody - Neuronal MarkerSee all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Rabbit polyclonal to 200 kD Neurofilament Heavy - Neuronal Marker
  • SpecificityThe antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).
  • Tested applicationsIHC-FoFr, ICC/IF, ICC, IHC-P, IHC-Fr, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Cat, Human, Pig
  • Immunogen

    Recombinant fragment (Cow).

Properties

Applications

Our Abpromise guarantee covers the use of ab8135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr
ICC/IF
ICC
IHC-P
IHC-Fr
WB
  • Application notesIF: 1/1000.
    IHC-P: 1/1000.
    IHC-Fr: 1/1000.
    IHC-FoFr: Use at an assay dependent dilution (PMID 19075249).
    WB: 1/10000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
    • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      modifications
      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Target information above from: UniProt accession P12036 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 200 kDa neurofilament protein antibody
      • KIAA0845 antibody
      • KIAA0845 antibody
      • NEFH antibody
      • NEFH antibody
      • Neurofilament H antibody
      • Neurofilament heavy polypeptide 200kD antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker images

    • The image showing results obtained after protease pretreatment, illustrating high definition neurofilament staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
    • ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.

      The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.

      The primary antibody was incubated for 1 hour at 25°C.

      Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.

      Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.

      Red = ab8135 staining 200kD Neurofilament

      See Abreview

    • ab8135 staining 200 kD Neurofilament Heavy in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).

      Tissue was fixed with formaldehyde, blocked with 5% serum for 1 hour at 25°C and permeabilzed with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) for 12 hours at 25°C. An Alexa Fluor®555 goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

      See Abreview

    References for Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker (ab8135)

    This product has been referenced in:
    • Ritter J  et al. Neonatal hyperoxia exposure disrupts axon-oligodendrocyte integrity in the subcortical white matter. J Neurosci 33:8990-9002 (2013). Read more (PubMed: 23699510) »
    • Liu G  et al. The effect of urine-derived stem cells expressing VEGF loaded in collagen hydrogels on myogenesis and innervation following after subcutaneous implantation in nude mice. Biomaterials 34:8617-29 (2013). Read more (PubMed: 23932297) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric buffer, pH6.0
    Sample Human Tissue sections (fetal spinal cord)
    Specification fetal spinal cord
    Permeabilization Yes - Tween-20
    Fixative Paraformaldehyde
    Username

    Mr. Heiko Locher

    Verified customer

    Submitted Sep 23 2013

    Application Immunocytochemistry
    Sample Human Cell (Kelly Neuroblastoma Cell line)
    Specification Kelly Neuroblastoma Cell line
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Neuro2a)
    Loading amount 6 µg
    Specification Neuro2a
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 22 2012

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 16 2012

    Application Western blot
    Sample Rat Cell lysate - whole cell (B50 Cell line)
    Loading amount 6 µg
    Specification B50 Cell line
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 12 2012

    Application Immunocytochemistry
    Sample Rat Cultured Cells (33B)
    Specification 33B
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 06 2012

    Application Immunocytochemistry
    Sample Mouse Cultured Cells (Neuro2a Cell Line)
    Specification Neuro2a Cell Line
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 06 2012

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Neuro2a)
    Specification Neuro2a
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 07 2012

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (B50 Neuroblastoma cell line)
    Specification B50 Neuroblastoma cell line
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 05 2012

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Retina)
    Specification Retina
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted May 28 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"