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Anti-200 kD Neurofilament Heavy antibody [RMO-24] (ab78038)

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Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody [RMO-24]See all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Mouse monoclonal [RMO-24] to 200 kD Neurofilament Heavy
  • SpecificityReacts with Neurofilament Heavy (NF-H), tail domain (phosphate dependent). Dephosphorylation of the NF-H will results in diminished immunoreactivity.
  • Tested applicationsWB, IP, ELISA, IHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Rat neurofilaments

  • EpitopeTail domain (Phosphate dependent)
  • Positive controlBrain

Properties

Applications

Our Abpromise guarantee covers the use of ab78038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
ELISA
IHC-P
IHC-Fr
  • Application notesIHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    The antibody has been used successfully on Bouin's and alcohol fixed paraffin embedded or frozen tissue sections. Formalin fixed paraffin embedded tissue sections require pretreatment with trypsin.
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 112 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
    • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      modifications      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

      • Target information above from: UniProt accession P12036 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        200 kDa neurofilament protein antibodyNEFH antibodyNeurofilament H antibody
        Neurofilament heavy polypeptide 200 kD antibodyNeurofilament heavy polypeptide 200 kDa antibodyNeurofilament heavy polypeptide 200kD antibodyNeurofilament heavy polypeptide 200kDa antibodyNeurofilament heavy polypeptide antibodyNeurofilament triplet H protein antibodyNF 200 antibodyNF H antibodyNF-H antibodyNF200 antibodyNFH antibodyNFH_HUMAN antibody
      see all

    References for Anti-200 kD Neurofilament Heavy antibody [RMO-24] (ab78038)

    ab78038 has not yet been referenced specifically in any publications.

    Publishing research using ab78038 ? Please let us know so that we can cite the reference in this datasheet

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