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Anti-200 kD Neurofilament Heavy antibody [RNF402] - Neuronal Marker (ab3966)

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Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody [RNF402] - Neuronal MarkerSee all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Mouse monoclonal [RNF402] to 200 kD Neurofilament Heavy - Neuronal Marker
  • SpecificityAxonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the 200 kDa neurofilament protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms.
  • Tested applicationsIHC-P, IHC-Fr, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Monkey
  • Immunogen

    Neurofilament preparation of calf brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab3966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/10 - 1/100.
IHC-Fr IHC-Fr: 1/10 - 1/100.
WB WB: 1/25 - 1/250.

Target

  • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.

    • Target information above from: UniProt accession P12036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      200 kDa neurofilament protein antibodyKIAA0845 antibodyKIAA0845 antibody
      NEFH antibodyNEFH antibodyNeurofilament H antibodyNeurofilament heavy polypeptide 200kD antibodyNeurofilament heavy polypeptide 200kDa antibodyNeurofilament heavy polypeptide antibodyNeurofilament triplet H protein antibodyNeurofilament triplet H protein antibodyNF H antibodyNF-H antibodyNFH antibodyNFH_HUMAN antibody
    see all

Anti-200 kD Neurofilament Heavy antibody [RNF402] - Neuronal Marker images

  • Immunohistochemistry (Frozen sections) - Anti-200 kD Neurofilament Heavy antibody [RNF402] - Neuronal Marker (ab3966)
    Immunohistochemistry (Frozen sections) - Anti-200 kD Neurofilament Heavy antibody [RNF402] - Neuronal Marker (ab3966)
    Immunohistochemistry on frozen section of rat brain

References for Anti-200 kD Neurofilament Heavy antibody [RNF402] - Neuronal Marker (ab3966)

ab3966 has not yet been referenced specifically in any publications.

Publishing research using ab3966 ? Please let us know so that we can cite the reference in this datasheet

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The isotype is IgM - what is the allotype?

Thank you for your enquiry. This antibody is IgM kappa, we don't have any additional information. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"