Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibodySee all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Chicken polyclonal to 200 kD Neurofilament Heavy
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length protein (purified) (Cow)

  • Positive control
    • Rat cerebral cortex lysate. Rat cortical neurons and glia.

Properties

Applications

Our Abpromise guarantee covers the use of ab72996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
ICC/IF 1/25000.

Target

  • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Target information above from: UniProt accession P12036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 200 kDa neurofilament protein antibody
    • NEFH antibody
    • Neurofilament H antibody
    • Neurofilament heavy polypeptide 200 kD antibody
    • Neurofilament heavy polypeptide 200 kDa antibody
    • Neurofilament heavy polypeptide 200kD antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF 200 antibody
    • NF H antibody
    • NF-H antibody
    • NF200 antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-200 kD Neurofilament Heavy antibody images

  • Anti-200 kD Neurofilament Heavy antibody (ab72996) at 1/50000 dilution + Rat cerebral cortex lysate

    Predicted band size : 115 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of rat cortical neurons and glia using ab72996 at 1/25000 dilution showing 200 kD Neurofilament Heavy staining (red).

References for Anti-200 kD Neurofilament Heavy antibody (ab72996)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 7% · Temperature: 25°C
Sample Mouse Cell (DRG neurons)
Specification DRG neurons
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
Username

Mr. Shachar Dagan

Verified customer

Submitted Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"