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Recombinant fragment (Cow).
Our Abpromise guarantee covers the use of ab8135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19075249|
|ICC||Use at an assay dependent concentration.|
ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.
The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.
The primary antibody was incubated for 1 hour at 25°C.
Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.
Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.
Red = ab8135 staining 200kD Neurofilament
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