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Recombinant fragment corresponding to Cow 200 kD Neurofilament Heavy (C terminal).
Our Abpromise guarantee covers the use of ab8135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19075249|
|ICC||Use at an assay dependent concentration.|
ab8135 staining 200 kD Neurofilament Heavy in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).
Tissue was fixed with formaldehyde, blocked with 5% serum for 1 hour at 25°C and permeabilzed with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) for 12 hours at 25°C. An Alexa Fluor® 555 goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.
Immunocytochemical staining of 33B cells (rat oligodendroglioma) with ab8135 at 1/1000. Cells were cultured in DMEM/10% FCS on coverslips in 12 well plates. Cells were washed with PBS and fixed in 4% PFA until staining. Cells were washed teice for 2 minutes and then treated with 3% hydrogen peroxide in methanol for 15 minutes at room temperature. The sample was blocked in 10% goat serum in 1% PBSA and then incubated with the primary antibody in 1% PBSA for 2 hours at room temperature. A biotinylated goat anti-rabbit was used as the secondary at 1/200 and then incubated with ABC for 30 minutes at room temperature, followed by DAB in PBS (brown). The sample was counter-stained with haematoxylin.
ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.
The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.
The primary antibody was incubated for 1 hour at 25°C.
Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.
Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.
Red = ab8135 staining 200kD Neurofilament
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