Anti-200 kD Neurofilament Heavy antibody [NF-01] (ab7795)

Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody [NF-01]See all 200 kD Neurofilament Heavy primary antibodies ...
  • Description
    Mouse monoclonal [NF-01] to 200 kD Neurofilament Heavy
  • SpecificityThis antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.
  • Tested applicationsELISA, IHC-Fr, ICC, IHC-P, WB, IHC - Wholemount, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
    Predicted to work with: a wide range of other species, all Mammals
  • Immunogen

    A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

Properties

Applications

Our Abpromise guarantee covers the use of ab7795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 200 kDa neurofilament protein antibody
    • KIAA0845 antibody
    • KIAA0845 antibody
    • NEFH antibody
    • NEFH antibody
    • Neurofilament H antibody
    • Neurofilament heavy polypeptide 200kD antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-200 kD Neurofilament Heavy antibody [NF-01] images

  • ab7795 at a dilution of 1/1000, staining 200 kD Neurofilament Heavy  (Alexa 488 secondary at 1/2000) in rat brain tissue (30µm thick coronal sections) on free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-200 kD Neurofilament Heavy antibody [NF-01] (ab7795)

This product has been referenced in:
  • Roher AE  et al. Subjects harboring presenilin familial Alzheimer's disease mutations exhibit diverse white matter biochemistry alterations. Am J Neurodegener Dis 2:187-207 (2013). Read more (PubMed: 24093083) »
  • Samara A  et al. Proteomics reveal rat hippocampal lateral asymmetry. Hippocampus 21:108-19 (2011). WB ; Rat . Read more (PubMed: 20020437) »

See all 4 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Rat Tissue (Dorsal root ganglion)
Specification Dorsal root ganglion
Username

Dr. Shlomo Tsuriel

Verified customer

Submitted Feb 06 2014

Thank you for your inquiry.

I can confirm that we do not have ab73273 in our catalog anymore.

I can suggest ab7795 as alternative. It also recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various speci...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Differentiated embryonal carcinoma cells)
Specification Differentiated embryonal carcinoma cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2009

Please refer the paper on the datasheet (Histochemistry 100, 495,1993) which features the use of this monoclonal antibody. "In similar preparations (the authors refer to pellet of brain proteins after cold depolymerization, described elsewhere in th...

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