Anti-200 kD Neurofilament Heavy antibody [RNF404] (ab8972)

Overview

  • Product nameAnti-200 kD Neurofilament Heavy antibody [RNF404]
    See all 200 kD Neurofilament Heavy primary antibodies
  • Description
    Mouse monoclonal [RNF404] to 200 kD Neurofilament Heavy
  • SpecificityReacts with the intermediate filament protein of periferal and central nerve cells, corresponding in Western blotting with the 200 kDa protein. Specific for the phosphorylated isoform.
  • Tested applicationsIHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Human, Xenopus laevis, Monkey
  • Immunogen

    Neurofilament preparation of calf brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab8972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
WB 1/100 - 1/500.
IHC-P 1/50 - 1/100.

Target

  • FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • 200 kDa neurofilament protein antibody
    • KIAA0845 antibody
    • KIAA0845 antibody
    • NEFH antibody
    • NEFH antibody
    • Neurofilament H antibody
    • Neurofilament heavy polypeptide 200kD antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-200 kD Neurofilament Heavy antibody [RNF404] images

  • Immunohistochemistry on paraffin section of human heart

References for Anti-200 kD Neurofilament Heavy antibody [RNF404] (ab8972)

ab8972 has not yet been referenced specifically in any publications.

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