Anti-200kDa + 160kDa Neurofilament antibody [SMI-310] (ab24570)

Overview

  • Product nameAnti-200kDa + 160kDa Neurofilament antibody [SMI-310]See all 200kDa + 160kDa Neurofilament primary antibodies ...
  • Description
    Mouse monoclonal [SMI-310] to 200kDa + 160kDa Neurofilament
  • SpecificityAb 24570 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a much lesser extent, with neurofilament M in most mammalian species. Immunocytochemically, Ab 24570 reacts with selected axons. The extend of reaction with axons is more restricted than that of any other antiphosphoneurofilaments. Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with Ab 24570 . Ab 24570 needs less phosphatase treatment of neurofilaments for abolition of reactivity than any other antibody to phosphorylated neurofilaments, suggesting that a very extensive degree of hyperphosphorylation of neurofilaments is necessary for its reactivity. In pathological conditions, reaction with Ab 24570 may be found also in neuronal cell bodies.
  • Tested applicationsIHC-Fr, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: all Mammals
  • Immunogen

    Homogenized hypothalmi recovered from Fischer 344 rats.

  • General notes


    In Alzheimer's disease, Ab 24570 provides a strong reaction with extraneuronal (ghost) neurofibrillary tangles. Intraneuronal tangles remain unreactive. Ab 24570 may react with an unusual and sparsely distributed form of phosphorylated neurofilaments that may be exacerbated in certain pathologic conditions. Ab 24570 distinguishes sporadic inclusion-body myositis from hereditary inclusion-body myopathies.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01M Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notes In Alzheimer's disease, Ab 24570 provides a strong reaction with extraneuronal (ghost) neurofibrillary tangles. Intraneuronal tangles remain unreactive. Ab 24570 may react with an unusual and sparsely distributed form of phosphorylated neurofilaments that may be exacerbated in certain pathologic conditions. Ab 24570 distinguishes sporadic inclusion-body myositis from hereditary inclusion-body myopathies.
  • Clonality Monoclonal
  • Clone numberSMI-310
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
WB 1/1000. Predicted molecular weight: 160-200 kDa.
IHC-P Use at an assay dependent dilution.
ELISA 1/1000.

Target

  • RelevanceNeurofilaments usually contain three intermediate filament proteins: L, M (160kDa), and H (200kDa) which are involved in the maintenance of neuronal caliber. 200kDa Neurofilament has an important function in mature axons that is not subserved by the two smaller Neurofilament proteins. There are a number of repeats of the tripeptide K S P, 200kDa Neurofilament is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of 200kDa Neurofilament results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NFM and NFH), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • 150kDa medium antibody
    • 160 kDa neurofilament protein antibody
    • 200 kDa neurofilament protein antibody
    • KIAA0845 antibody
    • NEF 3 antibody
    • NEF3 antibody
    • NEFH antibody
    • NEFM antibody
    • Neurofilament 3 antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament medium polypeptide 150kDa antibody
    • Neurofilament medium polypeptide antibody
    • Neurofilament triplet H protein antibody
    • Neurofilament triplet M protein antibody
    • Neurofilament3 antibody
    • NF H antibody
    • NF M antibody
    • NFH antibody
    • NFM antibody
    see all

References for Anti-200kDa + 160kDa Neurofilament antibody [SMI-310] (ab24570)

This product has been referenced in:
  • Boomkamp SD  et al. The development of a rat in vitro model of spinal cord injury demonstrating the additive effects of rho and ROCK inhibitors on neurite outgrowth and myelination. Glia 60:441-56 (2012). WB ; Rat . Read more (PubMed: 22139636) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab24570.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"