Anti-200kDa + 160kDa Neurofilament antibody [SMI-33] (ab24569)

Overview

  • Product nameAnti-200kDa + 160kDa Neurofilament antibody [SMI-33]See all 200kDa + 160kDa Neurofilament primary antibodies ...
  • Description
    Mouse monoclonal [SMI-33] to 200kDa + 160kDa Neurofilament
  • SpecificityAb24569 reacts with a nonphosphorylated epitope accessible in both, phosphorylated and nonphosphorylated neurofilament H and, to a lesser extent, in neurofilament M. In contrast to other antinonphosphoneurofilaments, reaction with isolated normal neurofilaments is not intensified by dephosphorylation.
  • Tested applicationsIHC-FoFr, ELISA, WB, ICC, IHC-P more details
  • Species reactivity
    Reacts with: Xenopus laevis
    Predicted to work with: all Mammals
  • Immunogen

    Homogenized hypothalmi recovered from Fischer 344 rats.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01M Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • PurityAscites
  • Clonality Monoclonal
  • Clone numberSMI-33
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr
ELISA
WB
ICC
IHC-P
  • Application notesELISA: 1/1000.
    ICC: Use at an assay dependent dilution. Post-fixation in cold methanol or methanol/hydrogen peroxide is recommended.
    IHC-P: 1/1000 (on tissues fixed with paraformaldehyde or formaldehyde-containing fixatives such as Bouin). Perform heat mediated antigen retrieval before commencing with IHC staining protocol (autoclaving deparaffinized sections for 10 min in distilled water or by boiling unembedded 0.5 cm thick blocks in Tris buffered saline, pH 9.0, in a microwave oven for 15 min).
    IHC-Fo: 1/1000. Use at an assay dependent dilution (on tissues perfusion fixed with paraformaldehyde or formaldehyde-containing fixatives such as Bouin). Post-fixation in cold methanol or methanol/hydrogen peroxide is recommended.
    Reaction is poor in material fixed in glutaraldehyde / paraformaldehyde, and in material treated with trypsin.

    WB: 1/1000. Predicted molecular weight: 160-200 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceNeurofilaments usually contain three intermediate filament proteins: L, M (160kDa), and H (200kDa) which are involved in the maintenance of neuronal caliber. 200kDa Neurofilament has an important function in mature axons that is not subserved by the two smaller Neurofilament proteins. There are a number of repeats of the tripeptide K S P, 200kDa Neurofilament is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of 200kDa Neurofilament results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NFM and NFH), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    • Cellular localizationCytoplasmic
    • Alternative names
      • 150kDa medium antibody
      • 160 kDa neurofilament protein antibody
      • 200 kDa neurofilament protein antibody
      • KIAA0845 antibody
      • NEF 3 antibody
      • NEF3 antibody
      • NEFH antibody
      • NEFM antibody
      • Neurofilament 3 antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament medium polypeptide 150kDa antibody
      • Neurofilament medium polypeptide antibody
      • Neurofilament triplet H protein antibody
      • Neurofilament triplet M protein antibody
      • Neurofilament3 antibody
      • NF H antibody
      • NF M antibody
      • NFH antibody
      • NFM antibody
      see all

    References for Anti-200kDa + 160kDa Neurofilament antibody [SMI-33] (ab24569)

    This product has been referenced in:
    • Banerjee P  et al. Stable expression and heterologous coupling of the kappa opioid receptor in cell lines of neural and nonneural origin. Life Sci 58:1277-84 (1996). Read more (PubMed: 8614281) »
    • Hewitt CD  et al. Aluminium maltol-induced neurocytoskeletal changes in fetal rabbit midbrain in matrix culture. Neuropathol Appl Neurobiol 17:47-60 (1991). Read more (PubMed: 2057050) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"