20x PBS Buffer with Tween 20 (ab64028)

Overview

  • Product name20x PBS Buffer with Tween 20
    See all PBS and Tween 20 reagents
  • Tested applicationsSuitable for: IHC-Pmore details
  • General notesIn order to provide this product at 20X, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution. This product is available for research use only, not for diagnostic or therapeutic work.

Properties

  • RelevancePBS (Phosphate Buffered Saline) and Tween 20 are used as a wash buffer in immunolabeling techniques. Tween 20 is added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.
  • Alternative names
    • PBS with Tween 20
    • Phosphate Buffered Saline and Tween 20
    • Phosphate Buffered Saline with Tween 20

Applications

Our Abpromise guarantee covers the use of ab64028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for 20x PBS Buffer with Tween 20 (ab64028)

ab64028 has not yet been referenced specifically in any publications.

Product Wall

Tween-20 is often used at 0.2%-0.5% as a membrane permeabilizing solution. At 0.1% in PBS, Tween-20 can help reduce background but does not have much effect for permeabilizing. Tween-20 may be found at many life science vendors.

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Thewashing / rinsing buffer to be used with this kit isPBS/Tween 20.

We do carry this buffer in our catalog in 2 different sizes(125 ml and 1000 ml):

ab64028: http://www.abcam.com/20x-PBS-Buff...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"