Overview

  • Product name20x PBS buffer with Tween 20
    See all PBS and Tween 20 reagents
  • Tested applicationsSuitable for: IHC-Pmore details
  • General notesIn order to provide this product at 20x, the salts in solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.

Properties

  • RelevancePBS (Phosphate Buffered Saline) and Tween 20 are used as a wash buffer in immunolabeling techniques. Tween 20 is added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies.
  • Alternative names
    • PBS with Tween 20
    • Phosphate Buffered Saline and Tween 20
    • Phosphate Buffered Saline with Tween 20

Applications

Our Abpromise guarantee covers the use of ab64247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for 20x PBS buffer with Tween 20 (ab64247)

This product has been referenced in:

See 1 Publication for this product

Product Wall

ab64247 is supplied as a liquid at a 20X concentration. To prepare a 500 mL solution of 1X PBS with 0.05% Tween-20, add 25 mL of the 20X stock to 475 mL ddH2O. To prepare a 1 L solution, add 50 mL of the 20X stock to 950 mL ddH2O. The 1X solutio...

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Thewashing / rinsing buffer to be used with this kit isPBS/Tween 20.

We do carry this buffer in our catalog in 2 different sizes(125 ml and 1000 ml):

ab64028: http://www.abcam.com/20x-PBS-Buff...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"