293T whole cell lysate (ab95494)

Overview

  • Product name
    293T whole cell lysate
  • General notes
    Description: Control cell lysates from 293T human kidney cells were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 µg of cellular protein was mixed with the same volume of 2X SDS-PAGE loading buffer.

    Growth Properties: adeherent Morphology: epithelial Organ: kidney Antigen Expression: SV40 T antigen Age: fetus


    The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line.

Properties

  • Form
    Lyophilised:Add 100 µl distilled water to the sample. Final concentration is 2 mg/ml.
  • Storage instructions
    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 5% Glycerol, 1% SDS, 0.002% Bromophenol blue, 31mM Tris HCl, pH 6.8
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Lysate notes
    The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line.

References

ab95494 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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