Overview

Properties

  • RelevanceHDL (high-density-lipoprotein), LDL (low-density lipoprotein) and VLDL (very low-density lipoprotein) are lipoproteins that act as carrier proteins for cholesterol. The regulation of HDL-cholesterol and LDL-cholesterol plays a central role in various disease development. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events.
  • Alternative names
    • High density lipoprotein
    • LDL
    • Low density lipoprotein
    • Very low density lipoprotein
    • VLDL
    see all

Applications

Our Abpromise guarantee covers the use of ab105138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

References for 2X LDL/VLDL Precipitation Buffer (ab105138)

This product has been referenced in:
  • Liu C  et al. Effects of a novel pharmacologic inhibitor of myeloperoxidase in a mouse atherosclerosis model. PLoS One 7:e50767 (2012). Functional Studies ; Mouse . Read more (PubMed: 23251382) »

See 1 Publication for this product

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This product does not contain phosphotungstic acid.

The precipitation method used on our HDL and LDL/VLDL Cholesterol Assay Kit (ab65390) is based on the use of a water-soluble non-ionic polymer. The primary advantage of our HDL precipitation buffer (ab105138) over dextran/Mg or other IP buffers is that...

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This assay was not designed with this type of downstream application in particular and we have not tested the miRNA and Lipid association after separation of HDL and LDL. It might be possible to isolate RNA but there are RNases which have not been inhi...

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Thank you for your enquiry.

This product (ab105138: HDL Precipitation Buffer) isone of the components of ab65390: HDL and LDL/VLDL Cholesterol Assay Kit.



The Protocol can be found on the product datasheet (ab65390) at thi...

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Thank you for your inquiry. Unfortunately, this buffer has only been tested to precipitate total HDL. If this buffer precipitates and in what efficiency the major subfractions of HDL, namely, HDL2 and HDL3, have not been specifically tested. U...

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Thank you for your patience while I contacted the lab for further information.  They have informed me that the fractions are separated into the HDL and the LDL/VLDL fractions. Hence the LDL and VLDL both will be present in the precipitate. Pease see se...

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Thank you for contacting Abcam. The buffers used in the separation of these fractions are very specific to separate the HDL and the LDL fractions. We cannot reveal the composition as it is proprietary and at this point we do not have the GE image of th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"