Overview

  • Product name
    3-Aminobenzamide (3-AB)
  • Description
    PARP inhibitor
  • Alternative names
    • 3-AB
  • Biological description
    Poly(ADP-ribose) polymerase (PARP) inhibitor (Ki = 1.8 µM). Displays various biological actions including stimulation of angiogenesis. Additionally displays neuroprotective, anti-necrotic effects and shows free-radical scavenging ability.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab141069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • MG-63 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of 3-aminobenzamide (3-AB) (ab 141069). Decreased expression of cyclin D1 (ab134175) in MG-63 cells correlates with an increase in 3-aminobenzamide (3-AB) concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab134175 at 1/500 dilution and ab8226 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

References

This product has been referenced in:
  • Strosznajder R & Gajkowska B Effect of 3-aminobenzamide on Bcl-2, Bax and AIF localization in hippocampal neurons altered by ischemia-reperfusion injury. the immunocytochemical study. Acta Neurobiol Exp (Wars) 66:15-22 (2006). Read more (PubMed: 16617673) »
  • Virág L & Szabó C The therapeutic potential of poly(ADP-ribose) polymerase inhibitors. Pharmacol Rev 54:375-429 (2002). Read more (PubMed: 12223530) »
  • Purnell MR & Whish WJ Novel inhibitors of poly(ADP-ribose) synthetase. Biochem J 185:775-7 (1980). Read more (PubMed: 6248035) »

See 0 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab141069.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

Sign up