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Anti-3-Nitrotyrosine antibody [11C2] (ab53232)

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1 question for ab53232

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Question 1

Wednesday 23-November-2011

Hello, Do any of these antibodies, anti-Oxoguanine 8, anti-Nitrotyrosine, and anti-Malondialdehyde, work well or somewhat adequate with half Karnovsky's fixative? Thanks

ANSWER:

 

Thank you for your enquiry.

I am sorry to confirm that non of our Oxoguanine 8, Nitrotyrosine, and Malondialdehyde antibodies have been tested with half Karnovsky's fixative. Regrettably, this means we therefore cannot confirm and guarantee how well this fixative would work with these antibodies.

I have been provided the following information from our sources:

ab64548 Oxoguanine 8 antibody [2Q2311] This antibody has been tested with a)  ice-cold methanol or b)  2% paraformaldehyde followed by ice-cold 70% ethanol.  A brief literature search did not turn up any references that used this clone with half Karnovsky's fixative.  

ab78163  Nitrotyrosine antibody [2A8.2] We have not tested this antibody with half Karnovsky's fixative.  Only 10% neutral buffer formalin has been used.

ab94883 Nitrotyrosine antibody [HM11] Not tested with Karnovsky's fixative        

ab53232 Anti-3-Nitrotyrosine antibody [11C2] Not tested with Karnovsky's fixative   

ab6463  Anti-Malondialdehyde antibody For all reference that we have, tissues fixation was done only with paraformaldehyde (4% is a standard concentration). Glutaraldehyde is not necessary.

The only antibody I have regrettably not yet been able to obtain more information on is ab61392  Anti-3-Nitrotyrosine antibody [39B6]. I would assume that this has not been tested in Karnovsky's, as this would be quite unusual for IHC testing. However, if I obtain any further details I will send these on to you as soon as possible.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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