3-Nitrotyrosine antibody [11C2] (ab53232)

Product Name 

3-Nitrotyrosine antibody [11C2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [11C2] to 3-Nitrotyrosine

Immunogen 

Chemical / Small Molecule: Nitrotyrosine conjugated to KLH.

Reacts with 
(species key)

N/A

Specificity 

This antibody reacts with free and bound Nitrotyrosine.

Tested applications 
(see key)

ELISA, ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/100 - 1/1000.
ICC: 1/100 - 1/500.
IHC-P: 1/250 (PMID 19541933).
WB: 1/100 - 1/500. Predicted molecular weight: 1 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

11C2

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for 3-Nitrotyrosine antibody [11C2] (ab53232)

  This product has been used in: (two most recent references)

McGrath-Morrow S et al. Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia. Am J Physiol Lung Cell Mol Physiol 296:L565-73 (2009). PubMed: 19151108

Podowski M et al. Complex integration of matrix, oxidative stress, and apoptosis in genetic emphysema. Am J Pathol 175:84-96 (2009). IHC-P. PubMed: 19541933

See all 3 publication references for this product.


If you publish research using ab53232 please let us know so that we can cite the reference on this datasheet.

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