Products:Signal Transduction >> Metabolism >> Amino Acids
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All lanes : Anti-3-Nitrotyrosine antibody [2A12] (ab52309) at 1 µg/ml
Lane 1 : mouse brain lysate
Lane 2 : rat brain lysate
Lysates/proteins at 40 µg per lane.
Observed band size : 25,55,160 kDa (why is the actual band size different from the predicted?)
A: Western Blot using ab52309 detected with Supersignal West Pico Substrate, 30 second exposure.
B: Same experiment blocked with buffer containing 1mM 3-Nitrosine.
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