Biological descriptionSelective PI3K inhibitor. Inhibits the initial phase of the autophagy. Blocks autophagosome formation. Potentiates apoptosis. Inhibits protein degradation (IC50 = 5 mM, rat hepatocytes).
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Tran AT et al. Autophagy inhibitor 3-methyladenine potentiates apoptosis induced by dietary tocotrienols in breast cancer cells. Eur J Nutr54:265-72 (2015).
Read more (PubMed: 24830781) »
Pliyev BK & Menshikov M Differential effects of the autophagy inhibitors 3-methyladenine and chloroquine on spontaneous and TNF-a-induced neutrophil apoptosis. Apoptosis17:1050-65 (2012).
Read more (PubMed: 22638980) »
Viola G et al. MG-2477, a new tubulin inhibitor, induces autophagy through inhibition of the Akt/mTOR pathway and delayed apoptosis in A549 cells. Biochem Pharmacol83:16-26 (2012).
Read more (PubMed: 21964343) »
Wu YT et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J Biol Chem285:10850-61 (2010).
Read more (PubMed: 20123989) »
Seglen PO & Gordon PB 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc Natl Acad Sci U S A79:1889-92 (1982).
Read more (PubMed: 6952238) »