Overview

  • Product nameAnti-3-Nitrotyrosine antibody [2A12]
    See all 3-Nitrotyrosine primary antibodies
  • Description
    Mouse monoclonal [2A12] to 3-Nitrotyrosine
  • SpecificityThis antibody reacts with 3-Nitrotyrosine
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Ubiquitous species reactivity.
  • Immunogen

    3-Nitrotyrosine conjugated to KLH

  • Positive control
    • Mouse and rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab52309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml.

Target

  • RelevanceProtein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
  • Alternative names
    • Nitrotyrosine antibody

Anti-3-Nitrotyrosine antibody [2A12] images

  • All lanes : Anti-3-Nitrotyrosine antibody [2A12] (ab52309) at 1 µg/ml

    Lane 1 : mouse brain lysate
    Lane 2 : rat brain lysate

    Lysates/proteins at 40 µg per lane.


    Observed band size : 25,55,160 kDa (why is the actual band size different from the predicted?)

References for Anti-3-Nitrotyrosine antibody [2A12] (ab52309)

This product has been referenced in:
  • Greco T  et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016). Read more (PubMed: 26661201) »
  • Ashraf MI  et al. A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury. Cell Commun Signal 12:6 (2014). WB . Read more (PubMed: 24423080) »

See all 10 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab52309.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"