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3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.
Our Abpromise guarantee covers the use of ab61392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20708681|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
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Blocked with 5% milk for 2 hours.
ab61392 staining 3-nitrotyrosine in Bouin's fixed paraffin-embedded backskin sections of transgenic mice.
Blocker: 5% goat serum in buffer. Buffer: 99% washing buffer (7g BSA and 222ul gelatin topped up to 1L dH2O), 1% heat inactivated goat serum. Primary Dilution: 1/100 for 1 hour at room temperature. Secondary: FITC-conjugated goat anti-mouse IgG, 1/50 for 1 hour at room temperature.
ab61392 staining of Nitrotyrosine residues at 1/100 in rat liver formaldehyde fixed sections three days after zymosan treatment. Staining was detected with a fluorescein secondary antibody and counterstained with DAPI.