For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
|Components||1 x 96 tests|
|10X 3-nitrotyrosine Detector Antibody||1 x 1ml|
|10X Blocking Buffer||1 x 6ml|
|10X HRP Label||1 x 1ml|
|20X Buffer||1 x 20ml|
|3-nitrotyrosine BSA standard||1 vial|
|Extraction Buffer||1 x 15ml|
|Microplate 96 antibody coated wells in 12 strips||1 unit|
|HRP Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab116691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|