Overview

  • Product name3-Nitrotyrosine ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 8 6.9%
    1 8 6.9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    8 ng/ml
  • Range
    8 ng/ml - 1000 ng/ml
  • Assay durationMultiple steps standard assay
  • Product overview

    ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.


     


    All species.

  • Notes

    Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab116691 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    3-Nitrotyrosine ELISA Kit images

    • Example standard curve.

    Protocols

    References for 3-Nitrotyrosine ELISA Kit (ab116691)

    This product has been referenced in:
    • Roy E  et al. Developing electrochemical sensor for point-of-care diagnostics of oxidative stress marker using imprinted bimetallic Fe/Pd nanoparticle. Talanta 132:406-15 (2015). Read more (PubMed: 25476325) »
    • Yao R  et al. Adiponectin protects against paraquat-induced lung injury by attenuating oxidative/nitrative stress. Exp Ther Med 9:131-136 (2015). Sandwich ELISA ; Mouse . Read more (PubMed: 25452788) »

    See all 5 Publications for this product

    Product Wall



    3-nitrotyrosine is a chemical formed in vivo by the reaction of tyrosine with nitrating oxidants, superoxide and nitric acid (http://www.ncbi.nlm.nih.gov/pubmed/1416975) with the chemical formula C9H10N2O5. It's structure does not change betw...

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    Thank you for your enquiry.



    Lung is a great sample to run with this assay!


    My general recommendation is to homogenize and then sonicate the tissue. Sonicate until the sample is not sticky - DNA released from ruptured...

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    The competitive ELISA is considered to be more sensitive than the sandwich ELISA. Both are compatible with your samples

    Please do not hesitate to contact us if you need any more advice or information. ...

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    Thank you for your reply.


    Our recommendation would be to collect samples using the extraction buffer provided with the kit. Without knowing the recipe of the buffer it is impossible to say whether or not it is compatible. Please let me kn...

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    Thank you for your reply.

    Regarding your results - can you provide the formulation of the lysis buffer you are using? As the controls and standards are working, I expect the problem is with the sample preparation. Can you also provide your dat...

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    Thank you for contacting Abcam regarding ab116691.

    Unfortunately we do not sell these components separately. The kits are designed to provide sufficient amounts of reagents for 1 96 well plate, so in order to acquire additional strips, you wou...

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    Thank you for your enquiry regarding ab116691.

    3-nitrotyrosine BSA standard is from purified BSA.

    I hope this information helps you, and please do not hesitate to contact us if you have further questions.

    Thank you for contacting us.

    Here is the response from the lab:

    As far as we know we haven’t tested this kiton plasma samples. There is no reason that it is not possible, but the question is primarily one of sensitivity (are ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"