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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - 350 aa isoform DKK3 antibody (ab2459)
Ab2459 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 350 aa isoform DKK3 antibody (ab2459)
ab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22ºC. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22ºC. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.
This image is courtesy of an anonymous Abreview
Dkk3 antibody for WB in Human (2459)
350 aa isoform DKK3 antibody for IHC-P in Human (2459)
350 aa isoform DKK3 antibody for WB in Human (2459)
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