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Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-Dkk3 antibody
See all Dkk3 products (3) ...
Description
Goat polyclonal to Dkk3
Specificity
This antibody is expected to recognise the 350 aa isoform only.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: AAAALLGGEEI, corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3.
AAAALLGGEE I
Positive control
293 lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Secreted
Signal Transduction >> Adapters >> Transmembrane
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.03 - 0.1 µg/ml.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Relevance
DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.
Cellular localization
Secreted
Alternative names
- Dickkopf 3 antibody
- Dickkopf homolog 3 antibody
- Dickkopf related protein 3 antibody
see all
Database links
- Entrez Gene: 27122 Human
- NCBI: 40548393 Human
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
Anti-Dkk3 antibody images:
Western blot - 350 aa isoform DKK3 antibody (ab2459)
Ab2459 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 350 aa isoform DKK3 antibody (ab2459)
ab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22°C. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-Dkk3 antibody (ab2459)
This product has been referenced in:
- Saeb-Parsy Ket al. MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3. Br J Cancer 99:663-9 (2008). WB; Human.Read more (PubMed: 18665176) »
See 1 publication for this product
Publishing research using ab2459? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"