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Western blot - 350 aa isoform DKK3 antibody (ab2459)
(enlarge)
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Product Name
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350 aa isoform DKK3 antibody
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See all 350 aa isoform DKK3 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to 350 aa isoform DKK3
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Immunogen
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Synthetic peptide: AAAALLGGEEI, corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3.
BLAST 'AAAALLGGEEI' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is expected to recognise the 350 aa isoform only.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Western Blot – Heterogeneous bands between 45-65 kDa seen in 293 lysate.
Recommended for use at 1.0-3.0µg/ml.
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Positive control
(see definition)
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293 lysate.
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Cellular localization
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Secreted
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Research areas
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Stem Cells >> Signaling Pathways >> Wnt >> Secreted
Signal Transduction >> Adapters >> Transmembrane
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Relevance
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DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 350 aa isoform DKK3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 350 aa isoform DKK3 antibody images, references, products related to ab2459 and other tools.
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350 aa isoform DKK3 antibody images:
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 Western blot - 350 aa isoform DKK3 antibody (ab2459)
Ab2459 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
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References for 350 aa isoform DKK3 antibody (ab2459)
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This product has been used in:
Saeb-Parsy K et al. MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3. Br J Cancer 99:663-9 (2008). PubMed: 18665176
If you publish research using ab2459 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 350 aa isoform DKK3
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350 aa isoform DKK3 antibody - more information
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Customer reviews (feedback) regarding 350 aa isoform DKK3 antibody |
Customer FAQs regarding 350 aa isoform DKK3 antibody |
Protocols for 350 aa isoform DKK3 antibody |
Price and availability of products related to 350 aa isoform DKK3 antibody |
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350 aa isoform DKK3 antibody - related products:
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350 aa isoform DKK3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about 350 aa isoform DKK3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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