Overview

  • Product name
    Anti-4 Hydroxynonenal antibody
    See all 4 Hydroxynonenal primary antibodies
  • Description
    Goat polyclonal to 4 Hydroxynonenal
  • Specificity
    This antibody specifically binds to HNE modified proteins.
  • Tested applications
    Suitable for: IHC-Fr, ELISA, WBmore details
  • Immunogen

    HNE conjugated to KLH

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 0.2% PBS, 0.435% Sodium chloride, 30% Glycerol
  • Concentration information loading...
  • Purification notes
    Antibody was purified by HNE modified Protein-Sepharose affinity column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24932583
ELISA 1/4000 - 1/32000.
WB 1/4000 - 1/32000. Predicted molecular weight: 10 kDa.

Target

  • Relevance
    Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody

References for Anti-4 Hydroxynonenal antibody (ab46544)

This product has been referenced in:
  • Smuder AJ  et al. Cervical spinal cord injury exacerbates ventilator-induced diaphragm dysfunction. J Appl Physiol (1985) 120:166-77 (2016). Read more (PubMed: 26472866) »
  • Calamaras TD  et al. The lipid peroxidation product 4-hydroxy-trans-2-nonenal causes protein synthesis in cardiac myocytes via activated mTORC1-p70S6K-RPS6 signaling. Free Radic Biol Med 82:137-46 (2015). Read more (PubMed: 25617592) »

See all 9 Publications for this product

Product Wall

Thank you for contacting us.

We unfortunately don’t have the blocking peptide-like product to pre-absorb this goat polyclonal HNE antibody to test the specificity of the antibody.

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Thank you for calling Abcam yesterday.

The antibody, goat anti-HNE (ab46544) will recognize proteins that have been modified with HNE. The protein size of band is depended on the size of the modified protein.

Please let me know if the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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