Overview

  • Product nameAnti-4 Hydroxynonenal antibody
    See all 4 Hydroxynonenal primary antibodies
  • Description
    Rabbit polyclonal to 4 Hydroxynonenal
  • SpecificitySpecifically binds to HNE modified proteins.
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, a wide range of other species
  • Immunogen

    HNE conjugated to BC.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.0146% EDTA, 0.44% Sodium chloride, 50% Glycerol, 1.23% Sodium phosphate
  • Concentration information loading...
  • Purification notesThis antibody was purified by an HNE modified Protein-Sepharose affinity column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24140865
WB Use at an assay dependent concentration. Can be blocked with Hydroxynonenal modified Bovine Serum Albumin (ab194193).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000 - 1/20000.

Target

  • RelevanceAldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
  • Cellular localizationCytoplasmic
  • Alternative names
    • 4 HNE antibody
    • 4-Hydroxy-2-Nonenal antibody

Anti-4 Hydroxynonenal antibody images

  • Image from Wang J et al., PLoS One. 2013;8(1):e53951. Fig 7(A).; doi: 10.1371/journal.pone.0053951.

    Frozen mouse cardiac tissue was homogenized with lysis buffer containing 50 mmol/L Tris-HCl (pH7.5), 5 mmol/L EDTA, 10 mmol/L EGTA, 1X cock tail protease inhibitor, 1X alkaline phosphatase inhibitor and 1X acid phophatase inhibitor, 50 ug/ml phenylmenthysulfonyl fluoride and 1.23 mg/ml Chaps. Extracts were centrifuged at 12000 rpm at 4°C for 15 minutes. 10 ug of the sample proteins was mixed with loading buffer (40 mmol/L Tris-HCl, pH 6.8, 1% SDS, 50 mmol/L DTT, 7.5% glycerol and 0.003% bromophenol blue and heated at 95°C for 5 minutes, and subjected to electrophoresis on a gradient gel (4% to 12%) at 120V. After electrophoresis, the protein was transferred to a PVDF membrane in a transfer buffer. The PVDF membrane was rinsed briefly in TBS buffer containing 50 mM Tris, 137 mM NaCl, pH 7.5 and blocked in buffer (5% milk with 0.5% BSA in TBST buffer (TBS buffer containing 0.1% tween 20) at room temperature for 1 hour. The membrane was then incubated with rabbit anti 4-hydroxy-2-noneal (4HNE) antibody at 1/3000 dilution at 4°C over night, followed by washing three times. The secondary antibody was incubated with the membrane for another one hour at room temperature. Finally the antigen-antibody complexes were visualized with use of an enhanced chemiluminescence kit. Anti-GAPDH (Abcam) was used for normalizing.

     

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling 4-Hydroxynonenal with ab46545 at a dilution of 1/50 incubated for 13 hours at 4°C. Heat mediated antigen retrival was performed with Tris-EDTA at pH 9.0. Permeabilzation was via 0.025% Triton X-100. Blocking was with ab93695 ABC kit at 1% for 20 minutes at room temperature. ab93695 detection kit was used for signal amplification.

    See Abreview

  • Immunohistochemical analysis of Human uterine tissue with intrauterine growth restriction, staining 4 Hydroxynonenal with ab46545 at 1/200 dilution. Staining was detected using AEC.

References for Anti-4 Hydroxynonenal antibody (ab46545)

This product has been referenced in:
  • Yu YH  et al. PKC-ALDH2 Pathway Plays a Novel Role in Adipocyte Differentiation. PLoS One 11:e0161993 (2016). WB, IP ; Mouse . Read more (PubMed: 27575855) »
  • Softic S  et al. Lipodystrophy Due to Adipose Tissue-Specific Insulin Receptor Knockout Results in Progressive NAFLD. Diabetes 65:2187-200 (2016). Read more (PubMed: 27207510) »

See all 31 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions Reduced Denaturing (12,5%)
Loading amount 70 µg
Specification Bone tissue (Tibia)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Sep 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization Yes - 0.025% Triton x-100
Specification Bone tissue (Femur)
Blocking step Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Sep 11 2015

Application Western blot
Sample Mouse Tissue lysate - whole (muscle tissue)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount 20 µg
Specification muscle tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: RT°C
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Submitted Jun 19 2015

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse liver, Rat lung)
Gel Running Conditions Reduced Denaturing (gel 12 %)
Loading amount 50 µg
Specification Mouse liver, Rat lung
Blocking step SlimFast as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 18°C
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Submitted May 05 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Feb 27 2015

As I am not experienced in 4-HNE research nor accepted method 4-HNE western blot band densitometry, I must defer to the abReviews and publications that external researches have used ab46545 in. I have listed these below:

abReviews:
http://...

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Thank you for contacting us.

HNE modified protein was used to produce ab46545 (anti-4 hydroxynonenal antibody). It recognizes the specific group in the proteins that had been modified with HNE.

I hope this information is helpful t...

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Thank you for contacting us. BC stands for Blue Carrier, a stable soluble carrier protein like KLH. Yes, this 4HNE antibody can work with any animal and is not species specific. The laboratory has tested with PFA fixation but has not compared different...

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If I understand correctly, you would like me to extend the expiration dates of the 4 codes that were issued xxxxxxxsince the customer has not placed his order?

As the codes are only valid 4 months after...

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Thank you for contacting us.

I am very pleased to hear that your customer would like to test our antibodies in rabbit. The codes below will give you the value of the product off your next order before the expiration date. To redeem this offer...

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1-10 of 40 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"