Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)
Key features and details
- Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal
- Suitable for: WB, IHC-P, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-4 Hydroxynonenal antibody [HNEJ-2]
See all 4 Hydroxynonenal primary antibodies -
Description
Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.
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Positive control
- Colorectal carcinoma cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 28th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 0.02% Potassium chloride, 0.02% Monobasic dihydrogen potassium phosphate, 0.0125% Sodium chloride, 0.115% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from serum-free cell culture medium by ammonium sulfate purification followed by protein A purification. -
Clonality
Monoclonal -
Clone number
HNEJ-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000.
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IHC-P | (3) |
1/25.
|
IHC-FoFr | (1) |
1/25.
|
Notes |
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WB
1/1000. |
IHC-P
1/25. |
IHC-FoFr
1/25. |
Target
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Relevance
Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress. -
Cellular localization
Cytoplasmic -
Alternative names
- 4 HNE antibody
- 4-Hydroxy-2-Nonenal antibody
Images
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All lanes : Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) at 1/1000 dilution
Lane 1 : BSA cell lysate at 0.5 µg
Lane 2 : BSA cell lysate at 1 µg
Lane 3 : 4-Hydroxynonenal (BSA) cell lysate at 0.5 µg
Lane 4 : 4-Hydroxynonenal (BSA) cell lysate at 1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa why is the actual band size different from the predicted?Western blot: Anti-4-HNE antibody [HNEJ-2] (ab48506) staining at 1/1000 dilution, shown in black. In Western blot, ab48506 was shown to bind specifically to 4-HNE. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) 5000 dilution.
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Elevated brain oxidative damage in ischemic 5xFAD mice
Formalin-fixed, paraffin-embedded mouse brain tissue (from non-trasngenic or 5xFAD transgenic animals) stained for 4 Hydroxynonenal using ab48506 at in immunohistochemical analysis.
Scale bar = 20μm. (A2) and (B2) are representative images of 4-HNE staining (Red) in brain cortex and CA1 region, respectively.
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ab48506 at a 1/25 dilution staining 4-Hydroxy-2-Nonenal in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 15 hours at +4°C. Fixed with formaldehyde, heat mediated antigen retrieval step performed using citrate buffer. Blocked using 5% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 594.
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ab15463 staining 4-Hydroxynonenal in the Mouse melanoma cancer tissue sections by IHC-FoFr (PFA perfusion fixed frozen Sections). Tissue samples were fixed with 4% Formalin, Antigen retrieval carried out heat mediation using anautoclave for 10 minutes, permeabilized using 0.1% SDS and blocked with 1% BSAfor 15 minutes at 25°C. The sample was incubated with primary antibody (1/25 in PBS + 1% BSA) at 4°C for 12 hours.An Alexa Fluor®488-conjugated Goat anti-mouse IgG(H+L) monoclonal(1/100) was used as the secondary antibody.
Datasheets and documents
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Datasheet download
References (158)
ab48506 has been referenced in 158 publications.
- Xu X et al. Increased ATF2 expression predicts poor prognosis and inhibits sorafenib-induced ferroptosis in gastric cancer. Redox Biol 59:102564 (2023). PubMed: 36473315
- Jo J et al. Protective Effects of Orexin A in a Murine Model of Cisplatin-Induced Acute Kidney Injury. J Clin Med 11:N/A (2022). PubMed: 36498769
- Guo J et al. Hepatocyte-specific TMEM16A deficiency alleviates hepatic ischemia/reperfusion injury via suppressing GPX4-mediated ferroptosis. Cell Death Dis 13:1072 (2022). PubMed: 36572666
- Cao L et al. Traditional Chinese medicine Lingguizhugan decoction ameliorate HFD-induced hepatic-lipid deposition in mice by inhibiting STING-mediated inflammation in macrophages. Chin Med 17:7 (2022). PubMed: 34983596
- Zhang X et al. Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma. Cancer Commun (Lond) 42:287-313 (2022). PubMed: 35184419