450 nm Stop Solution for TMB Substrate (ab171529)
Overview
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Product name
450 nm Stop Solution for TMB Substrate
See all ELISA stop solution reagents -
Tested applications
Suitable for: ELISAmore details -
General notes
Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.
Product Use:
Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at Room Temperature. -
Storage buffer
pH: 1
Constituent: 5% Methanesulphonic acid -
Concentration information loading...
Associated products
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
- Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.
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Notes |
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ELISA
Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab171529 has been referenced in 17 publications.
- Pinkaew D et al. Fortilin interacts with TGF-ß1 and prevents TGF-ß receptor activation. Commun Biol 5:157 (2022). PubMed: 35197550
- Mendoza-Salazar I et al. Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library. Antibodies (Basel) 11:N/A (2022). PubMed: 35225871
- Fingerhut L et al. Ex Vivo and In Vitro Analysis Identify a Detrimental Impact of Neutrophil Extracellular Traps on Eye Structures in Equine Recurrent Uveitis. Front Immunol 13:830871 (2022). PubMed: 35251020
- Mao L et al. Novel Synthetic Lipopeptides as Potential Mucosal Adjuvants Enhanced SARS-CoV-2 rRBD-Induced Immune Response. Front Immunol 13:833418 (2022). PubMed: 35356002
- de Buhr N et al. Insights Into Immunothrombotic Mechanisms in Acute Stroke due to Vaccine-Induced Immune Thrombotic Thrombocytopenia. Front Immunol 13:879157 (2022). PubMed: 35619694