450 nm Stop Solution for TMB Substrate (ab171529)

Overview

  • Product name
    450 nm Stop Solution for TMB Substrate
    See all ELISA stop solution reagents
  • Tested applications
    Suitable for: ELISAmore details
  • General notes

    Abcam’s 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.

    Product Use:
    Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

    Storage Instructions:
    Rich Text Editor, strBody, Press ALT 0 for help

    The supplier’s product sheet says 'Product should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C short term (1-2 weeks). Store at +4°C.
  • Storage buffer
    Constituents: 90% Water, 10% Proprietary component
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab171529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

References

ab171529 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

At least one hour.

They have greater signal stability at the high end of the standard curve, which increases dynamic range and reproducibility.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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