46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)

Overview

  • Product name
    46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate
  • General notes
    Aliquots are at a concentration of 1-2 mg/ml and contain between 100ug and 200ug of lysate, with a minimum of 100ug. To run on SDS-PAGE under reducing conditions it is necessary for the end user to add reducing agent. Addition of 5% beta-mercaptoethanol is recommended (5ul beta-mercaptoethanol per 100ul lysate).
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    RIPA buffer (Sigma) with added protease inhibitors (Sigma). Nupage 4X LDS sample buffer (Invitrogen) added. The lysates have been boiled. Buffer does not contain a reducing agent.
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Purification notes
    Cell lysate from feeder free mouse embryonic stem cells selected for Sox1 expression.
  • Research areas
  • Background
    46C is a cell line, constructed in the laboratory of Austin Smith, in which a drug resistance gene is placed under the control of a Sox1 promoter. The cells used in this lysate were Sox1-positive neural progenitor cells selected using puromycin following growth of Mouse Embryonic Stem Cells in N2B27 neural differentiation media. The Sox1 selection technology is patented.

Associated products

Applications

Our Abpromise guarantee covers the use of ab27205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

References for 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)

ab27205 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

We do not sell cell lines, only lysates. Unfortunately I do not know where you can source 46C cell lines. You may try searching online for stem cell providers or consult the literature for which labs have access to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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