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Overview

  • Product nameAnti-5-Hydroxymethyluridine antibody
  • Description
    Goat polyclonal to 5-Hydroxymethyluridine
  • Tested applicationsIP, ELISA more details
  • Immunogen

    5-Hydroxymethyluridine coupled to a carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab19735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at an assay dependent concentration. PubMed: 22514282
ELISA ELISA: 1/25000.

Target

  • Relevance5 Hydroxymethyluridine is formed from thymidine during the oxidation of DNA by ionizing radiation or reactive oxygen species (ROS) that are formed from inflammatory response. This causes genetic mutations, altered gene expression and chromosomal instability that has generally been implicated with aging, cancers and autoimmune disease.
  • Alternative names
      5 HmdU antibody5 Hydroxymethyl 2’ deoxyuridine antibody

References for Anti-5-Hydroxymethyluridine antibody (ab19735)

This product has been referenced in:
  • Cliffe LJ  et al. JBP1 and 2 are Fe2+/2-OG dependent dioxygenases regulating the hydroxylation of thymidine residues in trypanosome DNA. J Biol Chem : (2012). IP . Read more (PubMed: 22514282) »

See 1 Publication for this product

Publishing research using ab19735 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Displaying 1 - 9 of 9 results for Abreviews and Q&A

10
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We purchased two 5-Hydroxymethyl-2’-deoxyuridine antibodies (both ab95259 and ab93155) from Abcam but none of them works on dot blot. We tried multiple conditions and there was simply no signal, contrary to what's posted on line (ab93155 is said to work o...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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We purchased two 5-Hydroxymethyl-2’-deoxyuridine antibodies (both ab95259 and ab93155) from Abcam but none of them works on dot blot. We tried multiple conditions and there was simply no signal, contrary to what's posted on line (ab93155 is said to work o...

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Thank you for contacting us.


While Dot Blot does indeed have a lot of similarity to a non-reduced/denatured western blot or with an indirect ELISA, we do consider it to be a separate application from either of these. We do however recogn...

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I have used ab93155 in a dot blot format to detect modified, pure genomic DNA but there is no signal. A colleague of mine has used your other 5-Hydroxymethyluridine antibody, ab19735 in the same experiment and has had good results. Meanwhile, my colleague...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab19735. To check the status of the order please contact our Customer Se...

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Im sorry I just noticed the signatures in the past emails, they were hidden at the bottom of the email below all of our correspondences. Our lab did indeed purchase ab93155 however we were never able to make it work. I used it at different dilutions and w...

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Thanks for your reply. I look forward to speaking with you.

Can I please have a direct email address or phone number to reply to, I already explained  my situation in a previous email. I feel as though some information was lost due my replies being sent to a generic abcam email.   We used the previous antibody for...

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Thanks for your reply. Actually you have just been corresponding with me regarding this issue. My name is Jeremy and my signature can be found at the bottom of these emails. While you have been sending emails to our general Scientific Support inbox at us....

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We certainly do guarantee our antibodies to work as noted on our online product datasheets. At this time ab19735 is described as having been tested in ELISA. So if the vial of ab19735 is not working in ELISA then I would be happy to send you a replacement...

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Thanks for your reply. I don't believe we discussed the details of your ELISA experiment using ab19735. For our records, please provide a few details regarding the experiment including the sample type and preparation methods and a brief description of the...

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To whom it may concern, I spoke to Jeremy in technical support a few days ago about antibody ab19735 (hydroxymethyl-uridine for blots) as I was having some difficulty with detection of the antibody. I additionally contacted another lab that uses this anti...

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Thank you for your enquiry. We certainly do guarantee our antibodies to work as noted on our online product datasheets. At this time ab19735 is described as having been tested in ELISA. So if the vial of ab19735 is not working in ELISA then I woul...

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I recently ordered the 5-hydroxymethyl uridine antibody from Abcam (ab19735), and am using for DNA on dot blots. While the antibody works very nicely with genomic DNA, oligos containing a single HMU residue are not recognized even when loaded in very high...

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Thank you for your enquiry. The immunogen used for Goat anti 5-Hydroxymethyluridine, antibody ab19735, was 5-HmUrd coupled to BSA. The 5-HbUrd immunogen was prepared as follows: The 5-HmUrd was dissolved in sodium periodate and reacted for 20 minu...

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Product code: 19735
Inquiry: Information about antibody 19735 Dear Sir, We are interested in detecting hydroxymethyluracil in oligonucleotides and would like a complement of information about the 19735 antibody. Does the antibody work exclusively on f...

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Thank you for contacting us.

The product will cross react as follows

5-Hydroxy-2-deoxyuridine (5-HmdUrd) 100%

5-Hydroxymethyluridine (5-HmUrd) 100%

Thymidine (T) 60%

Hydroxymethyluracil (HmU) 43%

Deoxy...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"