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Anti-5 Lipoxygenase antibody (ab39347)

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Overview

Product name

Anti-5 Lipoxygenase antibody
See all 5 Lipoxygenase products (6) ...

Description

Rabbit polyclonal to 5 Lipoxygenase

Specificity

This antibody is specific for 5 Lipoxygenase and BSA. It does not recognise 12-LO and 15-LO.

Tested applications

ICC/IF, WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Human, Pig

Predicted to work with

Cow

Immunogen

Synthetic peptide: QHRRKELETRQKQYRWMEWN conjugated to BSA, corresponding to amino acids 130/149 of Human/Rat 5 Lipoxygenase

QHRRKELETR QKQYRWMEWN

Positive control

Porcine leukocyte supernatant.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Whole serum, Tris buffered saline, pH 7.4

Concentration

Concentration information loading...

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase antibody (ab39347)Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase antibody (ab39347) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Pathway

Lipid metabolism; leukotriene A4 biosynthesis.

Sequence similarities

Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.

Post-translational
modifications

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Cellular localization

Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Target information above from: UniProt accessionP09917 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 5 LO antibody
  • 5 LO antibody
  • 5 LOX antibody
  • 5-lipoxygenase antibody
  • 5-LO antibody
  • 5LOX antibody
  • 5LPG antibody
  • ALOX 5 antibody
  • ALOX5 antibody
  • Arachidonate 5 lipoxygenase antibody
  • Arachidonate 5-lipoxygenase antibody
  • Arachidonic acid 5 lipoxygenase antibody
  • Leukotriene A4 synthase antibody
  • LOG 5 antibody
  • LOG5 antibody
  • LOX5_HUMAN antibody
  • MGC163204 antibody
see all

Anti-5 Lipoxygenase antibody images:

  Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase antibody (ab39347)

Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase antibody (ab39347)

ab39347 staining 5 Lipoxygenase in human monocytes (from apheresis) by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton-X100, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab39347 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.

See Abreview

References for Anti-5 Lipoxygenase antibody (ab39347)

This product has been referenced in:

  • Chakrabarti SKet al. Evidence for activation of inflammatory lipoxygenase pathways in visceral adipose tissue of obese Zucker rats. Am J Physiol Endocrinol Metab 300:E175-87 (2011). WB, IHC-P; Rat.Read more (PubMed: 20978234) »
  • Yang Het al. Chemopreventive effects of early-stage and late-stage supplementation of vitamin E and selenium on esophageal carcinogenesis in rats maintained on a low vitamin E/selenium diet. Carcinogenesis 32:381-8 (2011). Rat.Read more (PubMed: 21186300) »

See all 3 publications for this product

Publishing research using ab39347? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"