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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-5 Lipoxygenase antibody
See all 5 Lipoxygenase products (6) ...
Description
Rabbit polyclonal to 5 Lipoxygenase
Specificity
This antibody is specific for 5 Lipoxygenase and BSA. It does not recognise 12-LO and 15-LO.
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Human, Pig
Predicted to work with
Cow
Immunogen
Synthetic peptide: QHRRKELETRQKQYRWMEWN conjugated to BSA, corresponding to amino acids 130/149 of Human/Rat 5 Lipoxygenase
QHRRKELETR QKQYRWMEWN
Positive control
Porcine leukocyte supernatant.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Whole serum, Tris buffered saline, pH 7.4
Concentration
Concentration information loading...
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
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Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase antibody (ab39347)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab39347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200
WB: 1/250 - 1/750.Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
IHC-P
IHC-P: 1/50 - 1/250.IHC-P: 1/50 - 1/250.
Target
Function
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Pathway
Lipid metabolism; leukotriene A4 biosynthesis.
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
Post-translational
modifications
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Cellular localization
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Target information above from: UniProt accessionP09917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5 LO antibody
- 5 LO antibody
- 5 LOX antibody
see all
Database links
- Entrez Gene: 240 Human
- Entrez Gene: 11689 Mouse
- Entrez Gene: 25290 Rat
- Omim: 152390 Human
- SwissProt: P51399 Hamster
- SwissProt: P09917 Human
- SwissProt: Q5JQ14 Human
- SwissProt: P48999 Mouse
see all
Anti-5 Lipoxygenase antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase antibody (ab39347)
ab39347 staining 5 Lipoxygenase in human monocytes (from apheresis) by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton-X100, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab39347 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
References for Anti-5 Lipoxygenase antibody (ab39347)
This product has been referenced in:
- Chakrabarti SKet al. Evidence for activation of inflammatory lipoxygenase pathways in visceral adipose tissue of obese Zucker rats. Am J Physiol Endocrinol Metab 300:E175-87 (2011). WB, IHC-P; Rat.Read more (PubMed: 20978234) »
- Yang Het al. Chemopreventive effects of early-stage and late-stage supplementation of vitamin E and selenium on esophageal carcinogenesis in rats maintained on a low vitamin E/selenium diet. Carcinogenesis 32:381-8 (2011). Rat.Read more (PubMed: 21186300) »
See all 3 publications for this product
Publishing research using ab39347? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"