Overview

  • Product nameAnti-5 Lipoxygenase antibodySee all 5 Lipoxygenase primary antibodies ...
  • Description
    Rabbit polyclonal to 5 Lipoxygenase
  • Tested applicationsICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    QHRRKELETRQKQYRWMEWN

    conjugated to BSA, corresponding to amino acids 130/149 of Human/Rat 5 Lipoxygenase

  • Positive control
    • Porcine leukocyte supernatant.

Properties

Applications

Our Abpromise guarantee covers the use of ab39347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/250 - 1/750. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
IHC-P 1/50 - 1/250.

Target

  • FunctionCatalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
  • PathwayLipid metabolism; leukotriene A4 biosynthesis.
  • Sequence similaritiesBelongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Post-translational
    modifications
    Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
  • Cellular localizationCytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 LO antibody
    • 5 LO antibody
    • 5 LOX antibody
    • 5-lipoxygenase antibody
    • 5-LO antibody
    • 5-LOX antibody
    • 5LOX antibody
    • 5LPG antibody
    • ALOX 5 antibody
    • ALOX5 antibody
    • Arachidonate 5 lipoxygenase antibody
    • Arachidonate 5-lipoxygenase antibody
    • arachidonic 5-lipoxygenase alpha-10 isoform antibody
    • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-p10 isoform antibody
    • Arachidonic acid 5 lipoxygenase antibody
    • Leukotriene A4 synthase antibody
    • LOG 5 antibody
    • LOG5 antibody
    • LOX5_HUMAN antibody
    • MGC163204 antibody
    see all

Anti-5 Lipoxygenase antibody images

  • ab39347 staining 5 Lipoxygenase in human monocytes (from apheresis) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton-X100, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab39347 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

  • Immunofluorescence analysis of U2-OS, staining 5 Lipoxygenase with ab39347.

    Cells were fixed with paraformaldehyde and blocked with 2% serum for 1 hour at 37°C. Samples were incubated with primary antibody (1/200 in 1% FCS in PBS) for 1 hour at 37°C. An AlexaFluor®488-conjugated chicken anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-5 Lipoxygenase antibody (ab39347)

This product has been referenced in:
  • Zhang H  et al. ROS-Mediated Autophagy Induced by Dysregulation of Lipid Metabolism Plays a Protective Role in Colorectal Cancer Cells Treated with Gambogic Acid. PLoS One 9:e96418 (2014). WB . Read more (PubMed: 24810758) »
  • Takahashi S  et al. Phospholipase A2 expression in coronary thrombus is increased in patients with recurrent cardiac events after acute myocardial infarction. Int J Cardiol 168:4214-21 (2013). Read more (PubMed: 23948114) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Treatment 2ppm ozone for 3weeks
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Dog Cell (Macrophages)
Specification Macrophages
Permeabilization Yes - Triton X-100 0,1% (in PBS)
Fixative Paraformaldehyde
Username

Dr. Guilherme Dias de Melo

Verified customer

Submitted Jul 23 2013

Thank you for submitting an Abreview of ab39347. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jun 21 2012

Application Immunocytochemistry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jan 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Thanks for your call today and for letting us know about the problem with ab39347. As we discussed, I am sending a free of charge vial of ab53514 on the order *** (on back order until October 10) and a vial of ab59341 on the order ***, which sh...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (H2452 Cell line)
Loading amount 20 µg
Specification H2452 Cell line
Gel Running Conditions Reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2-OS)
Specification U2-OS
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"