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Overview

  • Product nameAnti-5 Lipoxygenase antibodySee all 5 Lipoxygenase primary antibodies ...
  • Description
    Rabbit polyclonal to 5 Lipoxygenase
  • SpecificityThis antibody is specific for 5 Lipoxygenase and BSA. It does not recognise 12-LO and 15-LO.
  • Tested applicationsICC/IF, WB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide: QHRRKELETRQKQYRWMEWN conjugated to BSA, corresponding to amino acids 130/149 of Human/Rat 5 Lipoxygenase

  • Positive controlPorcine leukocyte supernatant.

Properties

Applications

Our Abpromise guarantee covers the use of ab39347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/200.
WB WB: 1/250 - 1/750. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
IHC-P IHC-P: 1/50 - 1/250.

Target

  • Alternative names
      5 LO antibody5 LO antibody5 LOX antibody
      5-lipoxygenase antibody5-LO antibody5-LOX antibody5LOX antibody5LPG antibodyALOX 5 antibodyALOX5 antibodyArachidonate 5 lipoxygenase antibodyArachidonate 5-lipoxygenase antibodyarachidonic 5-lipoxygenase alpha-10 isoform antibodyarachidonic 5-lipoxygenase delta-10-13 isoform antibodyarachidonic 5-lipoxygenase delta-13 isoform antibodyarachidonic 5-lipoxygenase delta-p10 isoform antibodyArachidonic acid 5 lipoxygenase antibodyLeukotriene A4 synthase antibodyLOG 5 antibodyLOG5 antibodyLOX5_HUMAN antibodyMGC163204 antibody
    see all

Anti-5 Lipoxygenase antibody images

  • ab39347 staining 5 Lipoxygenase in human monocytes (from apheresis) by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton-X100, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab39347 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

  • Immunofluorescence analysis of U2-OS, staining 5 Lipoxygenase with ab39347.

    Cells were fixed with paraformaldehyde and blocked with 2% serum for 1 hour at 37°C. Samples were incubated with primary antibody (1/200 in 1% FCS in PBS) for 1 hour at 37°C. An AlexaFluor®488-conjugated chicken anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-5 Lipoxygenase antibody (ab39347)

This product has been referenced in:
  • Chakrabarti SK  et al. Evidence for activation of inflammatory lipoxygenase pathways in visceral adipose tissue of obese Zucker rats. Am J Physiol Endocrinol Metab 300:E175-87 (2011). WB, IHC-P ; Rat . Read more (PubMed: 20978234) »
  • Yang H  et al. Chemopreventive effects of early-stage and late-stage supplementation of vitamin E and selenium on esophageal carcinogenesis in rats maintained on a low vitamin E/selenium diet. Carcinogenesis 32:381-8 (2011). Rat . Read more (PubMed: 21186300) »

See all 3 Publications for this product

Product Wall

Displaying 1 - 7 of 7 results for Abreviews and Q&A

Thank you for submitting an Abreview of ab39347. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
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Verified customer

Submitted Jun 21 2012

Application Immunocytochemistry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Verified customer

Submitted Jan 16 2012

Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Verified customer

Submitted Nov 16 2011

Thanks for your call today and for letting us know about the problem with ab39347. As we discussed, I am sending a free of charge vial of ab53514 on the order *** (on back order until October 10) and a vial of ab59341 on the order ***, which shoul...

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Application Western blot
Sample Human Cell lysate - whole cell (H2452 Cell line)
Loading amount 20 µg
Specification H2452 Cell line
Gel Running Conditions Reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Verified customer

Submitted Sep 28 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2-OS)
Specification U2-OS
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Verified customer

Submitted Jul 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"