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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-5 Lipoxygenase antibody
See all 5 Lipoxygenase products (6) ...
Description
Rabbit polyclonal to 5 Lipoxygenase
Specificity
ab59341 detects endogenous levels of total 5 lipoxygenase protein.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthesized non phosphopeptide derived from human 5 lipoxygenase around the phosphorylation site of serine 271 (QLSPLE).
Positive control
Human skeletal muscle tissue (IHC) HuvEc cytoplasmic lysate (WB)
General notes
Store at -20° for 1 year
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 78 kDa.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Pathway
Lipid metabolism; leukotriene A4 biosynthesis.
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
Post-translational
modifications
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Cellular localization
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Target information above from: UniProt accessionP09917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5 LO antibody
- 5 LOX antibody
- 5-lipoxygenase antibody
see all
Database links
- Entrez Gene: 240 Human
- Entrez Gene: 11689 Mouse
- Omim: 152390 Human
- SwissProt: P09917 Human
- SwissProt: Q5JQ14 Human
- SwissProt: P48999 Mouse
- SwissProt: Q3TB75 Mouse
- SwissProt: Q3TBK8 Mouse
see all
Anti-5 Lipoxygenase antibody images:
Western blot - 5 Lipoxygenase antibody (ab59341)
All lanes : Anti-5 Lipoxygenase antibody (ab59341) at 1/500 dilution
Lane 1 : Extracts from HuvEc cells without immunizing peptide
Lane 2 : Extracts from HuvEc cells containing immunizing peptide
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - 5 Lipoxygenase antibody (ab59341)
ab59341, at a 1/50 dilution, staining human 5 Lipoxygenase in skeletal muscle in the presence and absence of the immunizing peptide, using Immunohistochemistry, Paraffin embedded tissue.
References for Anti-5 Lipoxygenase antibody (ab59341)
This product has been referenced in:
- Jun JI & Lau LF The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing. Nat Cell Biol 12:676-85 (2010). WB; Human.Read more (PubMed: 20526329) »
See 1 publication for this product
Publishing research using ab59341? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"