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Anti-5 Lipoxygenase (phospho S271) antibody (ab59395)

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Overview

Product name

Anti-5 Lipoxygenase (phospho S271) antibody
See all 5 Lipoxygenase products (6) ...

Description

Rabbit polyclonal to 5 Lipoxygenase (phospho S271)

Specificity

This detects endogenous levels of 5 Lipoxygenase only when phosphorylated at serine 271.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthesized phosphopeptide derived from human 5 Lipoxygenase around the phosphorylation site of serine 271.

Positive control

IHC-P: Human skeletal muscle tissue. WB: Extracts from HuvEc cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Energy Metabolism

  • Western blot - 5 Lipoxygenase (phospho S271) antibody (ab59395)Western blot - 5 Lipoxygenase (phospho S271) antibody (ab59395) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab59395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 70 kDa (predicted molecular weight: 78 kDa).

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Pathway

Lipid metabolism; leukotriene A4 biosynthesis.

Sequence similarities

Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.

Post-translational
modifications

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Cellular localization

Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Target information above from: UniProt accessionP09917 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 5 LO antibody
  • 5 LOX antibody
  • 5-lipoxygenase antibody
  • 5-LO antibody
  • 5LOX antibody
  • 5LPG antibody
  • ALOX 5 antibody
  • ALOX5 antibody
  • Arachidonate 5 lipoxygenase antibody
  • Arachidonate 5-lipoxygenase antibody
  • Arachidonic acid 5 lipoxygenase antibody
  • Leukotriene A4 synthase antibody
  • LOG 5 antibody
  • LOG5 antibody
  • LOX5_HUMAN antibody
  • MGC163204 antibody
see all

Database links

see all

Anti-5 Lipoxygenase (phospho S271) antibody images:

  Western blot - 5 Lipoxygenase (phospho S271) antibody (ab59395)

Western blot - 5 Lipoxygenase (phospho S271) antibody (ab59395)

All lanes : Anti-5 Lipoxygenase (phospho S271) antibody (ab59395) at 1/500 dilution

Lane 1 : Extracts from HuvEc cells.
Lane 2 : Extracts from HuvEc cells plus phosphopeptide.


Predicted band size : 78 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-5 Lipoxygenase (phospho S271) antibody (ab59395)

ab59395 has not yet been referenced specifically in any publications.

Publishing research using ab59395? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"