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All lanes : Anti-5 Lipoxygenase (phospho S523) antibody (ab30573) at 1/1000 dilution
Lane 1 : rat cortex lysate
Lane 2 : rat cortex lysate
Predicted band size : 78 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Lane 2 was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5Lipoxygenase
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