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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-5 Lipoxygenase (phospho S523) antibody
See all 5 Lipoxygenase products (6) ...
Description
Rabbit polyclonal to 5 Lipoxygenase (phospho S523)
Specificity
Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots of rat hippocampal lysates
Tested applications
Cross reactivity
Reacts with
Rat, Human
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho523 of human 5-Lipoxygenase (5-LO).
Positive control
Rat cortex lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - 5 Lipoxygenase (phospho S523) antibody (ab30573)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
The immunolabeling is completely eliminated by treatment with lambda-Phosphatase.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Pathway
Lipid metabolism; leukotriene A4 biosynthesis.
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
Post-translational
modifications
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Cellular localization
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Target information above from: UniProt accessionP09917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5 LO antibody
- 5 LO antibody
- 5 LOX antibody
see all
Database links
- Entrez Gene: 240 Human
- Entrez Gene: 25290 Rat
- Omim: 152390 Human
- SwissProt: P09917 Human
- SwissProt: Q5JQ14 Human
- SwissProt: P12527 Rat
- Unigene: 89499 Human
- Unigene: 9662 Rat
Anti-5 Lipoxygenase (phospho S523) antibody images:
Western blot - 5 Lipoxygenase (phospho S523) antibody (ab30573)
All lanes : Anti-5 Lipoxygenase (phospho S523) antibody (ab30573) at 1/1000 dilution
Lane 1 : rat cortex lysate
Lane 2 : rat cortex lysate
Predicted band size : 78 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Lane 2 was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser523 5Lipoxygenase
References for Anti-5 Lipoxygenase (phospho S523) antibody (ab30573)
ab30573 has not yet been referenced specifically in any publications.
Publishing research using ab30573? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"