Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805)

1 vial (out of 3) with no liquid inside (after centrifugation) - should be 50 ul

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not contain the amount as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Antibody is not binding in a gel shift or dot blot assay with purchased methyl cytidine DNA.

ANSWER:

Thank you for your call today and for letting us know about the trouble with ab10805.

As we discussed, I'm sending a free of charge vial of ab10805 on the order ***. This should ship out tomorrow and arrive on Monday.

Please keep me updated about the results using this replacement vial. If the results do not improve, I will be happy to help you further to resolve the issue. Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Dear Tech support

One of our customers wishes to know if antibodies ab10805, ab106918 are suitable ad guaranteed for Vibritome processed and PFA fixated tissue samples?

Please advise.

Thank you for your kind help.

Regards,

ANSWER:

Thank you for contacting us.


Anti-5-Methyl Cytidine antibody (ab10805) has been tested and is guaranteed in paraffin embedded and frozen section immunohistochemistry. Anti-5-hydroxymethyl Cytidine antibody (ab106918) has only been tested in frozen sections and would be guaranteed for this.


We have no information on if they will work with Vibritome processed sections. If your customer would like, I can issue a testing discount for ab106918 to be used in immunohistochemical staining of paraffin embedded tissue sections. In order to process this I will need their email address and the institution they belong to.


I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

In case the customer has already done 5 ul aliquots, what should he do now? Thank you again,  

ANSWER:

Thank you for your recent message.

I would suggest performing the experiments, though higher working concentration may be needed to get the expected results. This is mainly due to the fact that smaller aliquots (5 ul) were prepared and no additional protein (i.e. BSA) is added to the storage buffer so a thin film layer may have been formed the internal surface of the tubes and some antibody molecules have adsorbed to it.

If you need any further assistance in the future, please do not hesitate to contact me.        

Is there a problem to store ab10805 in aliquots of 5 ul? Thank you,

ANSWER:

Thank you for your enquiry.  

Generally, the size of the aliquots will depend on how much one typically uses in an experiment. Aliquots should be no smaller than 10 µl; the smaller the aliquot, the more the stock concentration is affected by evaporation and adsorption of the antibody onto the surface of the storage vial.   For further information regarding storage and aliquotting, please visit this site: http://www.abcam.com/index.html?pageconfig=resource&rid=10795  

If you need anything further or any help then please let me know.