Potent DNMT1 inhibitor. Induces DNA hypomethylation.
Potent DNA methyltransferase 1 (DNMT1) inhibitor (IC50 = 0.2 μM). Incorporated into DNA, inhibits DNMT1 activity to induce DNA hypomethylation. Interferes with protein synthesis. Shows antimetabolic and anticancer activities in vitro. Active in vivo.
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in water to 50 mM and in DMSO to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
References for 5-Aza-2'-deoxycytidine (Decitabine) (ab120842)
This product has been referenced in:
Shin DY et al. Decitabine, a DNA methyltransferase inhibitor, induces apoptosis in human leukemia cells through intracellular reactive oxygen species generation. Int J Oncol41:910-8 (2012).
Read more (PubMed: 22767021) »
Rodríguez-Reyes R & Morales-Ramírez P The in vivo induction of sister chromatid exchange by the demethylating agent 5-aza-2'-deoxycytidine. Mutagenesis26:551-4 (2011).
Read more (PubMed: 21454325) »
Liu QY et al. Decitabine, independent of apoptosis, exerts its cytotoxic effects on cell growth in melanoma cells. Environ Toxicol Pharmacol32:423-9 (2011).
Read more (PubMed: 22004962) »
Cortvrindt R et al. 5-Azacytidine and 5-aza-2'-deoxycytidine behave as different antineoplastic agents in B16 melanoma. Br J Cancer56:261-5 (1987).
Read more (PubMed: 2444244) »