The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000.
1/1000 - 1/2000. Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over ~ 20 minutes. Note: If needed, low levels of glutaraldehyde (0.1 - 0.3%) may be used in conjugation with paraformaldehyde.
RelevanceTryptophan is an amino acid occurring naturally in food. In the brain, it is converted to 5-hydroxy-tryptophan (5-HTP), which, in turn, is converted to serotonin. Serotonin is a neurotransmitter essential to regulating appetite, sleep, mood and pain levels.