Overview

  • Product nameAnti-5 Lipoxygenase antibody
    See all 5 Lipoxygenase primary antibodies
  • Description
    Goat polyclonal to 5 Lipoxygenase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Hamster
  • Immunogen

    Synthetic peptide:

    C-ERNKKKQLPYYYLSPD

    , corresponding to C terminal amino acids 651-666 of Human 5 Lipoxygenase

  • Positive control
    • Rat stomach lysate or human bone marrow lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesPeptide ELISA: antibody detection limit dilution 1:32,000.

    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
    • PathwayLipid metabolism; leukotriene A4 biosynthesis.
    • Sequence similaritiesBelongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Post-translational
      modifications
      Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
    • Cellular localizationCytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5 LO antibody
      • 5 LOX antibody
      • 5-lipoxygenase antibody
      • 5-LO antibody
      • 5-LOX antibody
      • 5LOX antibody
      • 5LPG antibody
      • ALOX 5 antibody
      • Alox5 antibody
      • Arachidonate 5 lipoxygenase antibody
      • Arachidonate 5-lipoxygenase antibody
      • arachidonic 5-lipoxygenase alpha-10 isoform antibody
      • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-p10 isoform antibody
      • Arachidonic acid 5 lipoxygenase antibody
      • Leukotriene A4 synthase antibody
      • LOG 5 antibody
      • LOG5 antibody
      • LOX5_HUMAN antibody
      • MGC163204 antibody
      see all

    Anti-5 Lipoxygenase antibody images

    • Anti-5 Lipoxygenase antibody (ab53514) at 0.3 µg/ml + Rat Stomach lysate (35µg protein in RIPA buffer).

      Predicted band size : 78 kDa
      Observed band size : 78 kDa

    References for Anti-5 Lipoxygenase antibody (ab53514)

    ab53514 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"