Overview

  • Product name
    Anti-5 Lipoxygenase antibody
    See all 5 Lipoxygenase primary antibodies
  • Description
    Goat polyclonal to 5 Lipoxygenase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Hamster
  • Immunogen

    Synthetic peptide:

    C-ERNKKKQLPYYYLSPD

    , corresponding to C terminal amino acids 651-666 of Human 5 Lipoxygenase

  • Positive control
    • Rat stomach lysate or human bone marrow lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab53514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1:32,000.

    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
    • Pathway
      Lipid metabolism; leukotriene A4 biosynthesis.
    • Sequence similarities
      Belongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Post-translational
      modifications
      Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
    • Cellular localization
      Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5 LO antibody
      • 5 LOX antibody
      • 5-lipoxygenase antibody
      • 5-LO antibody
      • 5-LOX antibody
      • 5LOX antibody
      • 5LPG antibody
      • ALOX 5 antibody
      • Alox5 antibody
      • Arachidonate 5 lipoxygenase antibody
      • Arachidonate 5-lipoxygenase antibody
      • arachidonic 5-lipoxygenase alpha-10 isoform antibody
      • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-p10 isoform antibody
      • Arachidonic acid 5 lipoxygenase antibody
      • Leukotriene A4 synthase antibody
      • LOG 5 antibody
      • LOG5 antibody
      • LOX5_HUMAN antibody
      • MGC163204 antibody
      see all

    Images

    • Anti-5 Lipoxygenase antibody (ab53514) at 0.3 µg/ml + Rat Stomach lysate (35µg protein in RIPA buffer).

      Predicted band size : 78 kDa
      Observed band size : 78 kDa

    References

    ab53514 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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