Recombinant
RabMAb

Anti-5 Lipoxygenase antibody [EP6072(2)] - BSA and Azide free (ab227991)

Overview

  • Product name
    Anti-5 Lipoxygenase antibody [EP6072(2)] - BSA and Azide free
    See all 5 Lipoxygenase primary antibodies
  • Description
    Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase - BSA and Azide free
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Positive control
    • Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.
  • General notes

    ab227991 is a PBS only buffer version of ab169755, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab169755 for information on protocols, dilutions, and image data.

Applications

Our Abpromise guarantee covers the use of ab227991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.

Target

  • Function
    Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
  • Pathway
    Lipid metabolism; leukotriene A4 biosynthesis.
  • Sequence similarities
    Belongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Post-translational
    modifications
    Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
  • Cellular localization
    Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 LO antibody
    • 5 LOX antibody
    • 5-lipoxygenase antibody
    • 5-LO antibody
    • 5-LOX antibody
    • 5LOX antibody
    • 5LPG antibody
    • ALOX 5 antibody
    • Alox5 antibody
    • Arachidonate 5 lipoxygenase antibody
    • Arachidonate 5-lipoxygenase antibody
    • arachidonic 5-lipoxygenase alpha-10 isoform antibody
    • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-p10 isoform antibody
    • Arachidonic acid 5 lipoxygenase antibody
    • Leukotriene A4 synthase antibody
    • LOG 5 antibody
    • LOG5 antibody
    • LOX5_HUMAN antibody
    • MGC163204 antibody
    see all

Images

  • This IHC data was generated using the same anti-5 Lipoxygenase antibody clone, EP6072(2), in a different buffer formulation (cat# ab169755).

    ab169755 staining 5 Lipoxygenase in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

References

This product has been referenced in:
  • Li C  et al. Qishen granules inhibit myocardial inflammation injury through regulating arachidonic acid metabolism. Sci Rep 6:36949 (2016). WB . Read more (PubMed: 27833128) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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