Anti-5 Lipoxygenase (phospho S271) antibody (ab59395)

Overview

  • Product nameAnti-5 Lipoxygenase (phospho S271) antibody
    See all 5 Lipoxygenase primary antibodies
  • Description
    Rabbit polyclonal to 5 Lipoxygenase (phospho S271)
  • SpecificityThis detects endogenous levels of 5 Lipoxygenase only when phosphorylated at serine 271.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human 5 Lipoxygenase around the phosphorylation site of serine 271.

  • Positive control
    • IHC-P: Human skeletal muscle tissue. WB: Extracts from HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab59395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 78 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
  • PathwayLipid metabolism; leukotriene A4 biosynthesis.
  • Sequence similaritiesBelongs to the lipoxygenase family.
    Contains 1 lipoxygenase domain.
    Contains 1 PLAT domain.
  • Post-translational
    modifications
    Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
  • Cellular localizationCytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 LO antibody
    • 5 LOX antibody
    • 5-lipoxygenase antibody
    • 5-LO antibody
    • 5-LOX antibody
    • 5LOX antibody
    • 5LPG antibody
    • ALOX 5 antibody
    • Alox5 antibody
    • Arachidonate 5 lipoxygenase antibody
    • Arachidonate 5-lipoxygenase antibody
    • arachidonic 5-lipoxygenase alpha-10 isoform antibody
    • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-13 isoform antibody
    • arachidonic 5-lipoxygenase delta-p10 isoform antibody
    • Arachidonic acid 5 lipoxygenase antibody
    • Leukotriene A4 synthase antibody
    • LOG 5 antibody
    • LOG5 antibody
    • LOX5_HUMAN antibody
    • MGC163204 antibody
    see all

Anti-5 Lipoxygenase (phospho S271) antibody images

  • All lanes : Anti-5 Lipoxygenase (phospho S271) antibody (ab59395) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells.
    Lane 2 : Extracts from HuvEc cells plus phosphopeptide.


    Predicted band size : 78 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-5 Lipoxygenase (phospho S271) antibody (ab59395)

ab59395 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The mouse 5-lipoxygenase, like the human protein is 77.9 kDa. You would therefore expect to see a band at around this size. However, this size can vary considerably depending on the cell lysate produced, how the gel is perf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"