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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes WB: 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
The immunolabeling is completely eliminated by treatment with lambda-Phosphatase.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Pathway Lipid metabolism; leukotriene A4 biosynthesis.
Sequence similarities Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Post-translational modifications Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Cellular localization Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Information by UniProt
5 LO antibody
5 LOX antibody
Anti-5 Lipoxygenase (phospho S523) antibody images
Western blot - 5 Lipoxygenase (phospho S523) antibody (ab30573)
References for Anti-5 Lipoxygenase (phospho S523) antibody (ab30573)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"