Anti-5 Lipoxygenase (phospho S523) antibody (ab30573)

Overview

  • Product nameAnti-5 Lipoxygenase (phospho S523) antibody
    See all 5 Lipoxygenase primary antibodies
  • Description
    Rabbit polyclonal to 5 Lipoxygenase (phospho S523)
  • SpecificitySpecific for the ~80k 5-LO phosphorylated at Ser523 in Western blots of rat hippocampal lysates
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phospho523 of human 5-Lipoxygenase (5-LO).

  • Positive control
    • Rat cortex lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab30573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
    The immunolabeling is completely eliminated by treatment with lambda-Phosphatase.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
    • PathwayLipid metabolism; leukotriene A4 biosynthesis.
    • Sequence similaritiesBelongs to the lipoxygenase family.
      Contains 1 lipoxygenase domain.
      Contains 1 PLAT domain.
    • Post-translational
      modifications
      Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
    • Cellular localizationCytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5 LO antibody
      • 5 LOX antibody
      • 5-lipoxygenase antibody
      • 5-LO antibody
      • 5-LOX antibody
      • 5LOX antibody
      • 5LPG antibody
      • ALOX 5 antibody
      • Alox5 antibody
      • Arachidonate 5 lipoxygenase antibody
      • Arachidonate 5-lipoxygenase antibody
      • arachidonic 5-lipoxygenase alpha-10 isoform antibody
      • arachidonic 5-lipoxygenase delta-10-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-13 isoform antibody
      • arachidonic 5-lipoxygenase delta-p10 isoform antibody
      • Arachidonic acid 5 lipoxygenase antibody
      • Leukotriene A4 synthase antibody
      • LOG 5 antibody
      • LOG5 antibody
      • LOX5_HUMAN antibody
      • MGC163204 antibody
      see all

    Anti-5 Lipoxygenase (phospho S523) antibody images

    • All lanes : Anti-5 Lipoxygenase (phospho S523) antibody (ab30573) at 1/1000 dilution

      Lane 1 : rat cortex lysate
      Lane 2 : rat cortex lysate


      Predicted band size : 78 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References for Anti-5 Lipoxygenase (phospho S523) antibody (ab30573)

    ab30573 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"