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Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)
![Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)](/ps/datasheet/images/ab10805_1.jpg)
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Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)
![Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)](/ps/datasheet/images/ab10805_2.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 5-Methyl Cytidine antibody [ 33D3] (ab10805)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 5-Methyl Cytidine antibody [ 33D3] (ab10805)](/ps/datasheet/images/ab10805_review_13478.jpg)
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Product Name
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5-Methyl Cytidine antibody [33D3]
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See all 5-Methyl Cytidine antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [33D3] to 5-Methyl Cytidine
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Immunogen
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Recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates.
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Specificity
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Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, MeDIP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)).
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. (see Hernandez-Blazquez, F. et al. (2000)).
IHC-Fr: Use at an assay dependent dilution.
MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila (see PMID 17845077).
While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunatly, we can no longer guarantee this antibody for use in ICC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA
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Relevance
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Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when cytosine is attached to a ribose ring (also known as a ribofuranose) via a ß-N1-glycosidic bond.
Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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33D3
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Thimerosal Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Storage in frost-free freezers is not recommended.
Should this product contain a precipitate microcentrifugation before use.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 5-Methyl Cytidine antibody [33D3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 5-Methyl Cytidine antibody [33D3] images, references, products related to ab10805 and other tools.
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5-Methyl Cytidine antibody [33D3] images:
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 Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)
ab10805 at 1/200 staining mouse bone marrow cells by ICC/IF. The cells were methanol fixed and blocked with BSA before incubation with the antibody for 1 hour. An Alexa Fluor ® 488 conjugated donkey anti-mouse antibody was used as the secondary. This image is courtesy of an Abreview submitted by Mr Albert Santamaria
See Abreview
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Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)
ab10805 at 1/200 staining Arabidopsis thaliana root tip meristematic cells by ICC/IF. A Cy3 conjugated sheep polyclonal antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr Manuela Silva
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 5-Methyl Cytidine antibody [ 33D3] (ab10805)
ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary. This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig
See Abreview
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References for 5-Methyl Cytidine antibody [33D3] (ab10805)
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This product has been used in: (two most recent references)
Gerlitz G & Bustin M Efficient cell migration requires global chromatin condensation. J Cell Sci 123:2207-17 (2010). ICC/IF. PubMed: 20530575
Ohta H et al. Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny. Biol Reprod 80:511-7 (2009). PubMed: 19073998
See all 4 publication references for this product.
If you publish research using ab10805 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 5-Methyl Cytidine
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5-Methyl Cytidine antibody [33D3] - more information
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Customer reviews (feedback) regarding 5-Methyl Cytidine antibody [33D3] |
Customer FAQs regarding 5-Methyl Cytidine antibody [33D3] |
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5-Methyl Cytidine antibody [33D3] - related products:
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5-Methyl Cytidine antibody [33D3] - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)](#ab10805_1.jpg)
![Immunocytochemistry/ Immunofluorescence - 5-Methyl Cytidine antibody [ 33D3] (ab10805)](#ab10805_2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 5-Methyl Cytidine antibody [ 33D3] (ab10805)](#ab10805_review_13478.jpg)
