Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805)

  Immunocytochemistry/ Immunofluorescence - Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805)

ab10805 at 1/200 staining mouse bone marrow cells by ICC/IF. The cells were methanol fixed and blocked with BSA before incubation with the antibody for 1 hour. An Alexa Fluor ® 488 conjugated donkey anti-mouse antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Mr Albert Santamaria

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  Immunocytochemistry/ Immunofluorescence - Anti-5-Methyl Cytidine antibody [33D3] - ChIP Grade (ab10805)

ab10805 at 1/200 staining Arabidopsis thaliana root tip meristematic cells by ICC/IF. A Cy3 conjugated sheep polyclonal antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Manuela Silva

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 5-Methyl Cytidine antibody [ 33D3] (ab10805)

ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.

This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig

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