Anti-5-Methyl Tetrahydrofolic Acid antibody [FA-24] (ab50564)


  • Product nameAnti-5-Methyl Tetrahydrofolic Acid antibody [FA-24]
  • Description
    Mouse monoclonal [FA-24] to 5-Methyl Tetrahydrofolic Acid
  • Specificityab50564 recognises 5MTHFA as free reagent, when conjugated to carrier such as KLH or BSA, or in the natural form in human plasma and serum when it is bound to the endogenous Folate Binder. It does not cross react with Tetrahydrofolic acid (THFA), Folinic acid (FNA), Dihydrofolic acid (FAH2, Citrovorum Factor, Leucovorin), nor with Vitamin B12.
  • Tested applicationsSuitable for: Competitive ELISA, Indirect ELISAmore details
  • Species reactivity
    not applicable
  • Immunogen

    5-Methyl Tetrahydrofolic Acid conjugated to KLH.

  • Epitopeab50564 reacts with an epitope present on the biologically active analog 5MTHFA but not on Folic acid.



Our Abpromise guarantee covers the use of ab50564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA
Indirect ELISA
  • Application notescELISA: Use at an assay dependent dilution.
    Note: Using a suboptimal dilution of the antibody it should be possible to detect as little as 1.0 ng/ml of 5MTH Folic Acid. Also, binding of ab50564 to 5MTH Folic acid-BSA coated on polyvinyl microtiter plate is inhibited by prior incubation of suboptimal dilution of the antibody with 5MTH Folic acid but not with Folic acid, Vitamin B12 or by BSA.

    I-ELISA: Use at an assay dependent dilution.
    Note: The minimum antibody titer of 1:5,000 was determined by Indirect ELISA using 1 µg/ml of 5MTH Folic acid- BSA conjugate for coating of microtiter plate. It has been noted that 5MTH Folic acid-BSA is being absorbed most effectivly on polyvinyl microtiter plates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVitamin B12 and folic acid are metabolically interrelated. The enzyme methionine synthetase, which catalyzes the conversion of homocysteine to methionine, requires vitamin B12 and 5MTHFA as cofactors. In the absence of vitamin B12, 5MTHFA cannot be converted to tetrahydrofolic acid and enter the metabolic pool of 1-carbon fragment acceptors. Since this is the only known metabolic pathway involving 5MTHFA in man, there will be a decrease in the availability of other folic acid derivatives required for various biosynthetic pathways. Vitamin B12 and folate deficiences are the most common causes of megaloblastic anemia, abnormal hemopoiesis, interference in the maintenance of normal nerve tissue and general intracellular uptake and function disorders in humans.
    • Alternative names
      • 5 Methyl Tetrahydrofolic Acid antibody
      • 5MTHFA antibody
      • Folic acid antibody

    References for Anti-5-Methyl Tetrahydrofolic Acid antibody [FA-24] (ab50564)

    ab50564 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your patience. The 5Me-THF-BSA conjugate was made using 5-methyl THF (M0132) conjugated to BSA. This can be done via the free amine group on the 5-methyl THF and amine groups on the BSA with a carbodiimide cross linker. The kit used t...

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