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Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
Our Abpromise guarantee covers the use of ab73938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunoblotting - detection of DNA with 5-methylocytosine on nitrocellulose. Use at 1/1000
|ICC/IF||1/50 - 1/100. Recommend fixing in 4% PFA followed by denaturation with 70% formamide in 2x SSC and chilling in 70%, 95%, and 99% EtOH for 2 min each. Diluting antibody in SSC (saline sodium citrate) may give optimal results. See protocols from PubMed: 11983892 and 17994007|
|Flow Cyt||1/100. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.|
ab73938 staining 5-Methyl Cytosine in human sperm cells by Flow Cytometry. Cells were fixed with 70% ethanol and permeabilized with 5% tween. The sample was incubated with the primary antibody (1/100 in PBST) for 30 minutes at 27°C. A Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/300) was used as the secondary antibody.