Anti-53BP1 (phospho S25) antibody (ab82550)

Overview

• Product nameAnti-53BP1 (phospho S25) antibodySee all 53BP1 primary antibodies ...
• Description
  Rabbit polyclonal to 53BP1 (phospho S25)
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Phosphorylated synthetic peptide corresponding to a portion of 53BP1 around serine 25 (NP_005648.1)

• Positive controlHuman colon adenocarcinoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAffinity purified using an epitope specific to Phospho 53BP1 (S25) immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • p53 pathway

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • BRCT Domain Proteins

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • p53

Applications

Target

• FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
• Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
• Sequence similaritiesContains 2 BRCT domains.
• Post-translational
  modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
  Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
• Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

Target information above from: UniProt accession Q12888 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7158 Human
  • Entrez Gene: 27223 Mouse
  • Omim: 605230 Human
  • SwissProt: Q12888 Human
  • SwissProt: P70399 Mouse
  • Unigene: 440968 Human
  • Unigene: 383499 Mouse
  • Unigene: 481841 Mouse

• Alternative names
  53 BP1 antibody53BP1 antibodyFLJ41424 antibody

  MGC138366 antibodyp202 antibodyp53 binding protein 1 antibodyp53 BP1 antibodyp53-binding protein 1 antibodyp53BP1 antibodyTP53 BP1 antibodyTP53B_HUMAN antibodyTP53BP1 antibodyTRP53 BP1 antibodyTumor protein 53 binding protein 1 antibodyTumor protein p53 binding protein 1 antibodyTumor suppressor p53 binding protein 1 antibodyTumor suppressor p53-binding protein 1 antibody

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Anti-53BP1 (phospho S25) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 53BP1 (phospho S25) antibody (ab82550)
  ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (mock phosphatase treated section), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 53BP1 (phospho S25) antibody (ab82550)
  ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (lambda and CIP phosphatase treated), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue,