Overview

  • Product nameAnti-53BP1 (phospho S25) antibodySee all 53BP1 primary antibodies ...
  • Description
    Rabbit polyclonal to 53BP1 (phospho S25)
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Phosphorylated synthetic peptide corresponding to a portion of 53BP1 around serine 25 (NP_005648.1)

  • Positive control
    • Human colon adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab82550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Target information above from: UniProt accession Q12888 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • TP53BP1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 (phospho S25) antibody images

  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (mock phosphatase treated section), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (lambda and CIP phosphatase treated), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue,

References for Anti-53BP1 (phospho S25) antibody (ab82550)

This product has been referenced in:
  • Roossink F  et al. The role of ATM and 53BP1 as predictive markers in cervical cancer. Int J Cancer 131:2056-66 (2012). IHC-P ; Human . Read more (PubMed: 22323184) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab82550.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"