For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human 53BP2/ASPP2.(Note: the amino acid sequence is proprietary; peptide available as ab36003.)
Our Abpromise guarantee covers the use of ab36004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 126 kDa).Can be blocked with Human 53BP2/ASPP2 peptide (ab36003).|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab36004 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab36004 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"