Overview

  • Product nameAnti-53BP1 antibody
    See all 53BP1 primary antibodies
  • Description
    Rabbit polyclonal to 53BP1
  • SpecificityRecognises 53BP1
  • Tested applicationsSuitable for: In situ hybridization, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to the carboxy-terminus of h53BP1, conjugated to a carrier protein.

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab21083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
In situ hybridization Use at an assay dependent concentration. PubMed: 23370393
WB Use at an assay dependent concentration. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).
ICC/IF 1/200. Used at a dilution of 1/200 for 30 min incubation (see Abreview submitted by Kirk McManus).
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 antibody images



  • Predicted band size : 220 kDa
    Western blot analysis of 53BP1 expression in HeLa whole cell lysate. Western blot analysis of 53BP1 expression in HeLa whole cell lysate.
  • ab21083 (2µg/ml) staining 53BP1 in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is ubiquitous nuclear staining throughout.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-53BP1 antibody (ab21083)

This product has been referenced in:
  • Han X  et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 1:e1500454 (2015). WB ; Human . Read more (PubMed: 26601238) »
  • Wang M  et al. EGFR-mediated chromatin condensation protects KRAS-mutant cancer cells against ionizing radiation. Cancer Res 74:2825-34 (2014). ICC/IF ; Human . Read more (PubMed: 24648348) »

See all 21 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (head and neck cancer)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (8)
Loading amount 15 µg
Treatment drug 24h
Specification head and neck cancer
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2015

Thank you very much for your reply.

That is no problem to arrange. I have organised a new order for you for a new vial of ab21083. This is on the order number xxxx(Purchase order numberxxxxx). Please let me know if you have any problems receiv...

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Thank you for taking time to complete our questionnaire. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

I appreciate the time you have spent in the labora...

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Thank you for contacting us yesterday and sorry for the delay in getting back to you.

I am sorry to hear that you have been having some difficulty in using the Anti-53BP1 antibody (ab21083). We take product complaints very seriously, and inves...

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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. However, I can tell you that it falls within the carboxy-terminus of h53BP1.
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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hearthe customer has haddifficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA)
Loading amount 15 µg
Specification MRC5VA
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (T lymphocyte)
Specification T lymphocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2010

Thank you for your enquiry. We do notice that there is some size discrepancy between the calculated MW vs. observed MW with the ab21083 antibody. This product has been tested in house on Hela cell lysate and the antigen that was used to gene...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jul 12 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"