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Synthetic peptide (C terminal).
Our Abpromise guarantee covers the use of ab21083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In situ hybridization||Use at an assay dependent concentration. PubMed: 23370393|
|WB||1/500 - 1/3000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).|
|ICC/IF||1/200. Used at a dilution of 1/200 for 30 min incubation (see Abreview submitted by Kirk McManus).|
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ICC/IF analysis using ab21083 at 1/1000 dilution labeling 53BP1 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 minutes. ab21083 detects 53BP1 protein at the nucleus (green).
Red: alpha Tubulin, a cytoskeleton marker.
5 % SDS-PAGE