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Synthetic peptide representing a portion of human 53BP1 encoded in part by exons 11 and 12 (LocusLink ID 7158).
Our Abpromise guarantee covers the use of ab36823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|Flow Cyt||Use a concentration of 1.5 µg/ml.
1.5 µg/ml in a 150 mcl reaction.
|WB||1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).|
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|