Overview

  • Product name
  • Description
    Rabbit polyclonal to 53BP1
  • Specificity
    This antibody is specific for 53BP1.
  • Tested applications
    Suitable for: IP, Flow Cyt, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide representing a portion of human 53BP1 encoded in part by exons 11 and 12 (LocusLink ID 7158).

  • Positive control
    • U205, HeLa, 293T, MO59K cell lysates (human) and MEF lysates (mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab36823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
Flow Cyt Use a concentration of 1.5 µg/ml.

1.5 µg/ml in a 150 mcl reaction.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
ICC/IF 1/100 - 1/500.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in disease
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling 53BP1 with ab36823 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-53BP1 antibody (ab36823) at 1/1000 dilution

    Lane 1 : U2OS cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Predicted band size : 214 kDa
    Observed band size : 220 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds
  • Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MEFs, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours.
  • Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MO59K cells, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours.
  • Flow Cytometry: 53BP1 Antibody [ab36823] - Flow cytometric detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 ab36823 in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).

References for Anti-53BP1 antibody (ab36823)

This product has been referenced in:
  • Kariolis MS  et al. Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies. J Clin Invest 127:183-198 (2017). ICC/IF ; Human . Read more (PubMed: 27893463) »
  • Pal D  et al. TGF-ß reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells. Elife 6:N/A (2017). ICC/IF ; Human . Read more (PubMed: 28092266) »

See all 49 Publications for this product

Product Wall

Thank you for getting back to me and for providing some further details of the experiments. There may be several factors which contribute to lower band on the blot.

Since proteinase inhibitors have been added to the lysis buffer, the chance o...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Feb 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
Yes - 0.5% Triton
Specification
MEF
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Jan 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
20 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 07 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Jul 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Human Cell (Colon cancer)
Specification
Colon cancer
Permeabilization
No
Fixative
Methanol
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Submitted Apr 14 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
Specification
Mouse Pancreatic cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 27 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Human Cell (U2OS osteosarcoma)
Specification
U2OS osteosarcoma
Permeabilization
No
Fixative
Formaldehyde
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Submitted Mar 20 2014

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (3-8% gradient)
Sample
Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification
U2OS osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF7)
Specification
MCF7
Fixative
Formaldehyde
Permeabilization
Yes - triton 1%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
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Submitted Apr 15 2013

1-10 of 13 Abreviews or Q&A

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