Overview

  • Product nameAnti-53BP1 antibody
    See all 53BP1 primary antibodies
  • Description
    Rabbit polyclonal to 53BP1
  • SpecificityThis antibody is specific for 53BP1.
  • Tested applicationsSuitable for: IP, Flow Cyt, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide representing a portion of human 53BP1 encoded in part by exons 11 and 12 (LocusLink ID 7158).

  • Positive control
    • U205, HeLa, 293T, MO59K cell lysates (human) and MEF lysates (mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab36823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
Flow Cyt Use a concentration of 1.5 µg/ml.

1.5 µg/ml in a 150 mcl reaction.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
ICC/IF 1/100 - 1/500.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similaritiesContains 2 BRCT domains.
  • Post-translational
    modifications
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all

Anti-53BP1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling 53BP1 with ab36823 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-53BP1 antibody (ab36823) at 1/1000 dilution

    Lane 1 : U2OS cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Predicted band size : 214 kDa
    Observed band size : 220 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds
  • Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MEFs, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours.
  • Ab36823, at a dilution of 1/100, staining 53BP1 foci in proliferating MO59K cells, both normal and exposed to 10 Gy of IR and double color immunofluorescence. Staining was for 2 hours.
  • Flow Cytometry: 53BP1 Antibody [ab36823] - Flow cytometric detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 ab36823 in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).

References for Anti-53BP1 antibody (ab36823)

This product has been referenced in:
  • Hollingworth R  et al. Activation of DNA Damage Response Pathways during Lytic Replication of KSHV. Viruses 7:2908-27 (2015). IF . Read more (PubMed: 26057167) »
  • Meena JK  et al. Telomerase abrogates aneuploidy-induced telomere replication stress, senescence and cell depletion. EMBO J 34:1371-84 (2015). IF . Read more (PubMed: 25820263) »

See all 26 Publications for this product

Product Wall

Thank you for getting back to me and for providing some further details of the experiments. There may be several factors which contribute to lower band on the blot.

Since proteinase inhibitors have been added to the lysis buffer, the chance o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Gel Running Conditions Reduced Denaturing (6%)
Loading amount 20 µg
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 07 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% Triton
Specification HeLa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Jul 05 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Human Cell (Colon cancer)
Specification Colon cancer
Permeabilization No
Fixative Methanol
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Submitted Apr 14 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
Specification Mouse Pancreatic cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 27 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Permeabilization No
Fixative Formaldehyde
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Submitted Mar 20 2014

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (3-8% gradient)
Sample Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7)
Specification MCF7
Fixative Formaldehyde
Permeabilization Yes - triton 1%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
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Submitted Apr 15 2013

Thank you for your inquiry.


I have checked our catalog regarding your search for an p53bpi antibody which binds in the
tudor domain of this protein.

Unfortunately, we have antibodies which will bind the N-or the C-Terminu...

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Thank you for your enquiry regarding ab36823.

Would you be so kind to label the MW markers on the image and to resend the picture to me? It would also help me if you could provide some further information about how the samples were prepared a...

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1-10 of 11 Abreviews or Q&A

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