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Synthetic peptide representing a portion of human 53BP1 encoded in part by exons 11 and 12 (LocusLink ID 7158).
Our Abpromise guarantee covers the use of ab36823 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|Flow Cyt||Use a concentration of 1.5 µg/ml.
1.5 µg/ml in a 150 mcl reaction.
|WB||1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).|
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|